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Biochemical Effect of Alpha-lipoic Acid and Insulin Alone and in Combination
on Changes in the Phospholipid Composition in Experimental Diabetic Neuropathy |
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Samy Ali Hussein,
Mamdouh El-Haggar,
Omayma Ahmed Abo-Zaid,
Mohammed Ragaa Hassanien and Ragab El-Shawarby |
Abstract:
Diabetic neuropathy is the most common complication of diabetes. We investigate
the effect of alpha-lipoic acid and insulin alone and in combination on changes
in the phospholipids composition in the sciatic nerve of experimental diabetic
neuropathy. A total of 120 male rats were used in this study. The experimental
induction of diabetes in rats was induced by a single intraperetinoel (i.p)
injection of 50 mg kg-1 of streptozotocin (STZ) freshly dissolved
in citrate buffer, pH 4.5. After eight weeks of diabetes induction all rats
were divided into six main equal groups, 20 animals each. Group I (control group):
Received no drugs, Group II (diabetic group), Group III (normal α-lipoic
acid-treated group), Group IV (diabetic alpha-lipoic acid -treated group), Group
V (diabetic insulin- treated group), Group VI (diabetic alpha-lipoic acid and
insulin-treated group). Eight weeks after diabetes induction therapeutic treatment
with alpha-lipoic acid (54 mg kg-1 b.wt. i.p daily) and insulin (2U
s.c daily) were given either alone or in combination and continued for six weeks.
Equivalent volumes of saline were given subcutaneously to the rats in the other
diabetic and non diabetic control groups. Blood samples and sciatic nerve tissues
were collected at 4 and 6 weeks from the onset of treatment for determination
of serum glucose and total cholesterol, total phospholipids and membrane phospholipids
composition of sciatic nerve. The obtained results revealed that, diabetic neuropathy
in rats resulted in marked increase in serum glucose level, sciatic nerve total
cholesterol and phosphatidylglycerol contents. Treatment with α-lipoic
acid significantly decreased serum glucose, phosphatidylglycerol and sphingomyelin
contents with increase in total cholesterol content in sciatic nerve. Insulin
treatment significantly increased total phospholipids and markedly decrease
phospholipids composition of rat sciatic nerve including phosphatidylethanolamine,
phosphatidylcholine, phosphatidylglycerol and sphingomyelin contents. Meanwhile,
treatment with α-lipoic acid and insulin combination significantly decreased
total phospholipids concentration and phospholipids composition in rat sciatic
nerve including phosphatidylcholine, phosphatidylglycerol and sphingomyelin
contents. These results indicate that, alterations in the amounts of phospholipids
composition in sciatic nerve could be related to the physiological changes of
early diabetic neuropathy. These result suggest that, administration of α-lipoic
acid combined with insulin prevent hyperglycemia-induced changes in phospholipids
composition suggesting its therapeutic potential in complications of diabetes
and dibetes neuropathy.
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How to cite this article:
Samy Ali Hussein, Mamdouh El-Haggar, Omayma Ahmed Abo-Zaid, Mohammed Ragaa Hassanien and Ragab El-Shawarby, 2013. Biochemical Effect of Alpha-lipoic Acid and Insulin Alone and in Combination
on Changes in the Phospholipid Composition in Experimental Diabetic Neuropathy. Asian Journal of Biological Sciences, 6: 242-256.
DOI: 10.17311/ajbs.2013.242.256
URL: https://scialert.net/abstract/?doi=ajbs.2013.242.256
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