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American Journal of Biochemistry and Molecular Biology
  Year: 2022 | Volume: 12 | Issue: 1 | Page No.: 1-12
DOI: 10.3923/ajbmb.2022.1.12
 
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Effects of Methanol Leaf Extract of Aristolochia bracteolata on Diethylnitrosamine Induced Hepatocellular Carcinoma in Rats

I.B. Gabriel , K. Christopher, H.A. Umaru and D. Dahiru

Abstract:
Background and Objective: Diethylnitrosamine (DEN) is a well-known liver carcinogen in rats, forming DNA adducts in the liver and inducing hepatocellular damage. The present study was focused on anticancer and antioxidant effects of methanol leaf extract of Aristolochia bracteolata and its fractions against diethylnitrosamine induced hepatocarcinoma in rats. Materials and Methods: Hepatocellular damage was induced using diethylnitrosamine orally. Hepatocellular damage was assessed using biochemical parameters of the liver function tests, antioxidant enzymes which are considered as biomarkers in hepatocarcinoma. Results: Diethylnitrosamine caused significant hepatocellular damage with liver enzymes (Apatite transaminases, alanine transaminases and alkaline phosphatase activities) of 381±0.84, 125±0.37, 510±0.51 and 293±1.45, 75±1.57, 274±1.71 compared to the control groups at p<0.05, respectively. However, treatments with methanol leaf extract of Aristolochia bracteolata and its fractions as well as Silymarin significantly reverse the liver damage 284±2.88, 298±4.26 298±1.57 for different doses of the plant extract and fractions and Silymarin 295±0.71, respectively. So also, a significant decrease was observed in total protein, albumin, superoxide dismutase, glutathione reductase, glutathione peroxidase and total antioxidant with a corresponding increase in total bilirubin, interestingly, Aristolochia bracteolata significantly increase total protein, albumin, superoxide dismutase, glutathione reductase, glutathione peroxidase and total antioxidant with a decrease in total bilirubin concentration. Conclusion: Generally, the of administration of methanol leaf extract of Aristolochia bracteolata and its fractions promotes the healing of the liver cells through the anti-proliferative, antioxidant and anti-inflammatory effects of this natural product by maintaining the hepatocellular membrane integrity.
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How to cite this article:

I.B. Gabriel, K. Christopher, H.A. Umaru and D. Dahiru, 2022. Effects of Methanol Leaf Extract of Aristolochia bracteolata on Diethylnitrosamine Induced Hepatocellular Carcinoma in Rats. American Journal of Biochemistry and Molecular Biology, 12: 1-12.

DOI: 10.3923/ajbmb.2022.1.12

URL: https://scialert.net/abstract/?doi=ajbmb.2022.1.12

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