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American Journal of Biochemistry and Molecular Biology
  Year: 2017 | Volume: 7 | Issue: 2 | Page No.: 53-64
DOI: 10.3923/ajbmb.2017.53.64
Morphological, Biochemical and Molecular Characterization of Alternaria brassicicola and Alternaria brassicae: A Comparative Overview
Sanjay Mishra , Ruchi Singh, Shailesh Kumar and Sudheer Kumar

Abstract:
Among different Brassica, Indian mustard (Brassica juncea), an important and predominant rabi oilseed crop of rapeseed mustard group in India, occupies 90% area of the total hectarage. Mustard encounters number of foliar diseases, among them Alternaria blight is the most devastating, causing yield loss up to 35-38%. Chemical properties as well as fatty acid profile govern oil superiority. Besides, the disease undesirably distresses seed quality by reducing seed size, seed discoloration and decline in oil content. In relevance, recently microsatellite locus ‘ABS28’ has been identified and characterized in terms of cross-transferability to other related and unrelated taxa. Data compiled authenticate that this monomorphic microsatellite marker is greatly particular for A. brassicicola, as no magnification signal has been observed from other closely related Alternaria species. No false positive or false negative analyses were witnessed with the assays showed the stringency of specific primer set ABS28F and ABS28R, consequently letting precise and sensitive revealing of A. brassicicola from cultures and specific plant samples. The assay was noticed to be sensitive, consistently detecting 0.01 ng of genomic DNA per PCR of the target fungi. Thus, marker allows assessing fungal growth from the initial phases of infection, providing a rapid and applied alternative to existing defined markers to differentiate and identify A. brassicicola from synchronously occurring fungus, A. brassicae and other fungi related to rapeseed mustard. Till date, 14 monomorphic and one polymorphic microsatellite markers have been recognized from the necrotrophic and phytopathogenic fungus A. brassicicola. Although, identified polymorphic marker (ABS1) prerequisites to be established to assist a further study on the genetic assembly of natural A. brassicicola populations and to match the diversity of isolates initiating from distinct host as well as geographical site. This review provides new insights into further exploration of primer set, ABS28F (50-GCTCCCACTCCTTCCGCGC-30) and ABS28R (50-GGAGGTGGAGTTACCGACAA-30) to probably amplify a more specific amplicon of 380 bp and to prove to be useful as an internal diagnostic marker to differentiate and analyze A. brassicicola from A. brassicae and other fungi associated with leaves, stem and siliquae of rapeseed-mustard.
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How to cite this article:

Sanjay Mishra, Ruchi Singh, Shailesh Kumar and Sudheer Kumar, 2017. Morphological, Biochemical and Molecular Characterization of Alternaria brassicicola and Alternaria brassicae: A Comparative Overview. American Journal of Biochemistry and Molecular Biology, 7: 53-64.

DOI: 10.3923/ajbmb.2017.53.64

URL: https://scialert.net/abstract/?doi=ajbmb.2017.53.64

 
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