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American Journal of Biochemistry and Molecular Biology
  Year: 2016 | Volume: 6 | Issue: 2 | Page No.: 45-52
DOI: 10.3923/ajbmb.2016.45.52
Characterization of Lipase Gene Fragment from Alcaligenes sp. JG3 Bacterium
Tri Joko Raharjo, Norman Yoshi Haryono, Dilin Rahayu Nataningtyas, Ery Nourika Alfiraza and Deni Pranowo

Abstract:
Objective: Degenerate PCR technique has been successfully used to identify a lipase gene fragment of Alcaligenes sp. JG3. Primers were designed based on lipase genes of bacterium Azospirillum sp. B510 and Alcaligenes faecalis using PrimerBlast software. Methodology: Sequence analysis of the fragment with a size of 0.4 kb amplified using the forward primer (AlF4) 5’-GTCTACAGCAATCCCAAGAC-3’ and reversed primer (AlR4): 5’-GGAGGGGTAAATCCACAGTT-3’ represented a 394 bp nucleotide sequence which has been submitted to NCBI GenBank with Accession No. of KP872319. Results: The obtained DNA sequence was confirmed as part of lipase gene as it shared 98% amino acid and 88.07% nucleotide similarity with lipase gene of Alcaligenes faecalis, that covered approximately 27% the gene. Conclusion: The designed primers based on lipase gene of Alcaligenes faecalis were able to amplify 394 bp lipase gene fragment of bacterial strain Alcaligenes sp. JG3.
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How to cite this article:

Tri Joko Raharjo, Norman Yoshi Haryono, Dilin Rahayu Nataningtyas, Ery Nourika Alfiraza and Deni Pranowo, 2016. Characterization of Lipase Gene Fragment from Alcaligenes sp. JG3 Bacterium. American Journal of Biochemistry and Molecular Biology, 6: 45-52.

DOI: 10.3923/ajbmb.2016.45.52

URL: https://scialert.net/abstract/?doi=ajbmb.2016.45.52

 
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