Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
American Journal of Biochemistry and Molecular Biology
  Year: 2013 | Volume: 3 | Issue: 1 | Page No.: 91-100
DOI: 10.3923/ajbmb.2013.91.100
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Purification and Physicochemical Characterization of Anti-Gardnerella vaginalis Bacteriocin HV6b Produced by Lactobacillus fermentum Isolate from Human Vaginal Ecosystem

Baljinder Kaur, Praveen Balgir, Bharti Mittu, Ashish Chauhan and Balvir Kumar

Abstract:
Anti-Gardnerella vaginalis, Lactobacillus fermentum bacteriocin HV6b producing strain was isolated from vaginal swabs of healthy, fecund females. It was identified, purified and further characterized physicochemically. The relative molecular mass of bacteriocin was found to be less than 7 kDa. Maximum bacteriocin production was observed at 37°C, pH 6.5 after 16-18 h of incubation Proteinase K and pepsin strongly inhibited bacteriocin production. The bacteriocin has purified by absorption desorption method and characterized by reverse phase liquid chromatography. It exhibited high thermal stability and showed inhibitory activity over wide range of pH. The study revealed the possibility of using bacteriocin for bio-medical use and the L. fermentum strain as probiotic.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Purification and Molecular Characterization of Two-Antimicrobial Peptides Produced by Lactobacillus plantarum DU10
  •    Thermal, Mechanical, Morphological and Flame Retardance Properties of DABA Modified Novolac/CFRP Composites
  •    Enhancing the Functional Properties of Sportswear Fabric based Carbon Fiber
  •    The Effect of Aesthetic Beauty Design on the Performance Properties of Jacquard Weft Knitted Fabrics
  •    Factors Influencing Semi-Solid Microstructure
How to cite this article:

Baljinder Kaur, Praveen Balgir, Bharti Mittu, Ashish Chauhan and Balvir Kumar, 2013. Purification and Physicochemical Characterization of Anti-Gardnerella vaginalis Bacteriocin HV6b Produced by Lactobacillus fermentum Isolate from Human Vaginal Ecosystem. American Journal of Biochemistry and Molecular Biology, 3: 91-100.

DOI: 10.3923/ajbmb.2013.91.100

URL: https://scialert.net/abstract/?doi=ajbmb.2013.91.100

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 

Curve Bottom