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American Journal of Biochemistry and Molecular Biology

Year: 2013 | Volume: 3 | Issue: 1 | Page No.: 71-80
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Authors

Md. Fakruddin

Md. Fakruddin

Abhijit Chowdhury

Abhijit Chowdhury

Zakir Hossain

Keywords

Research Article

Competitiveness of Polymerase Chain Reaction to Alternate Amplification Methods

Md. Fakruddin Md. Fakruddin's LiveDNA, Abhijit Chowdhury Abhijit Chowdhury's LiveDNA and Zakir Hossain
PCR is the first method developed for amplification of DNA/nucleic acids in vitro. It was the method of choice since it's invent. PCR has many limitations which gave birth to alternative methods such as Loop Mediated Isothermal Amplification (LAMP), Nucleic Acid Sequence Based Amplification (NASBA), Self Sustained Sequence Replication (3SR), Rolling Circle Amplification (RCA) etc. A comprehensive review of the literature on the principles, limitations, challenges, improvements and alternatives of PCR was performed. Though PCR encountered some limitation afterwards and a no of technically improved alternatives has been devised worldwide, it still holds its standings as a gold standard for nucleic acid amplification preventing it from being obsolete. PCR has gone through some phenomenal modifications making continuous improvement of this method. Although, latter techniques offer better sensitivity and advantages where PCR has limitations, it is still commonly applied in the field of molecular biology for its extensive information all over the world, availability of reagents and methods, widespread validation and very good technical understanding among researchers. PCR is not obsolete despite of threats faced from other methods; rather it is continuously being modified to overcome the limitations. It will remain one of the ultimate methods of choice especially in developing countries for long.
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