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Detection of Canine Parvo Virus by Polymerase Chain Reaction Assay and its Prevalence in Dogs in and Around Mathura, Uttar Pradesh, India |
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Deepti Singh,
Amit Kumar Verma,
Amit Kumar,
Mukesh Srivastava,
Shanker Kumar Singh,
Arvind Kumar Tripathi,
Ashish Srivastava and Iftekhar Ahmed |
Abstract:
The early detection of the Canine Parvo Virus (CPV) is of paramount importance.
The present study was aimed to know the molecular epidemiology of Canine parvo
virus. Canine faecal samples from 100 dogs showing the clinical signs of gastroenteritis
in and around Mathura, Uttar Pradesh, India were collected and DNA was extracted
by phenol-chloroform method. CPV vaccine strain was used as a positive control.
Polymerase Chain Reaction (PCR) was carried out to amplify VPI/VP2 gene using
a set of 20-mer primers [pCPV-RT (Forward): 5’-CAT
TGG GCT TAC CAC CAT TT-3’; (Reverse):
5’-CCA ACC TCA GCT GGT CTC AT-3’)]
from position 3136-3155 to 3276-3295 of VP1/VP2 gene. A PCR product of approximately
160 bp was generated with positive faecal samples and CPV vaccine strain. After
screening, 63 dogs were found positive for CPV but no sex variation was noticed
amongst the CPV positive cases. Dogs, of the age group of ≤6 months were
more susceptible in comparison to of >6 months and highest occurrence was
noted in unvaccinated dogs and dogs in co-habitation with other dogs. Breed
wise distribution of CPV in dogs revealed that the prevalence of CPV was the
highest in Doberman (77.78%), followed by Spitz (78.57%), German shepherd (70.00%),
Labrador (68.75%), Pomeranian (45.45%). It is concluded that CPV is prevalent
in the Mathura and nearby area and it is more common in pups of age less than
6 months old and more prevalent in German shepherd, Labrador and Pomeranian
breeds of dog.
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How to cite this article:
Deepti Singh, Amit Kumar Verma, Amit Kumar, Mukesh Srivastava, Shanker Kumar Singh, Arvind Kumar Tripathi, Ashish Srivastava and Iftekhar Ahmed, 2013. Detection of Canine Parvo Virus by Polymerase Chain Reaction Assay and its Prevalence in Dogs in and Around Mathura, Uttar Pradesh, India. American Journal of Biochemistry and Molecular Biology, 3: 264-270.
DOI: 10.3923/ajbmb.2013.264.270
URL: https://scialert.net/abstract/?doi=ajbmb.2013.264.270
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