Research Article

Insights into Steviol Glycoside Biosynthesis Pathway Enzymes Through Structural Homology Modeling

Steviol glycosides are the major secondary metabolites synthesized through steviol glycoside biosynthesis pathway operating in the leaves of Stevia rebaudiana. Present article documents the structural analysis of enzymes specific to steviol glycoside biosynthesis pathway, kaurenoic acid-13 hydoxylase (KAH) and three UDP-glycosyltransferases (UGT85C2, UGT74G1 and UGT76G1). The in silico protein structure prediction server SWISS MODEL was used to predict and evaluate the models. The secondary structure data of predicted model for KAH was in accordance to that of cytochrome P450s suggesting its nativeness to the respective superfamily. Similarly, the secondary structure data of target UGTs also showed conservation with the structural information of glycosyltransferases superfamily. PROCHECK and QMEAN Z-score evaluations suggested that the models predicted for the 4 query enzymatic proteins were of good quality. In addition, Ligand binding site analysis and molecular docking analysis was carried out for the predicted models. The following data suggested a possibility of the presence of an alternate pathway for the synthesis of steviol glycosides.

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Praveen Guleria and Sudesh Kumar Yadav, 2013. Insights into Steviol Glycoside Biosynthesis Pathway Enzymes Through Structural Homology Modeling. American Journal of Biochemistry and Molecular Biology, 3: 1-19.

DOI: 10.3923/ajbmb.2013.1.19

URL: https://scialert.net/abstract/?doi=ajbmb.2013.1.19

American Journal of Biochemistry and Molecular Biology

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Praveen Guleria

Sudesh Kumar Yadav

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Research Article

Insights into Steviol Glycoside Biosynthesis Pathway Enzymes Through Structural Homology Modeling

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