Optimizing Sterilization Condition for the Initiation of Sweet Potato (Ipomoea batatas) Culture in vitro


	Asian Journal of Biotechnology
	Year: 2014 \| Volume: 6 \| Issue: 2 \| Page No.: 25-37 DOI: 10.3923/ajbkr.2014.25.37

Optimizing Sterilization Condition for the Initiation of Sweet Potato (Ipomoea batatas) Culture in vitro

R. Hammond, J. N. Buah, P.A. Asare and S. Acheampong

Abstract: This study is aimed at investigating the most efficient and cost effective sterilization condition for initiation of sweet potato culture in-vitro. Different concentrations of common or local household bleach (Power zone and Dettol) and non-readily available mercuric chloride as well as variation in their duration for sterilization were used to sterilize sweet potato buds to study their effectiveness in preventing microbial growth in sweet potato culture. The most common microbial contaminants observed in the sweet potato culture were fungi (Fusarium sp., Aspergillus nger, Penicillium sp. and Neurospora crassa). Mericuric chloride in combination with other treatment was found to be the most effective in suppressing microbial contaminants. Dettol in combination with other treatments was found to be ineffective in suppressing the microbial contamination. Duration of 20 min treatment was found to be the most effective in suppressing the microbial contamination but was very toxic to the explants. Treating explants for 10 min (control) was found to be ineffective in suppressing the microbial contamination but non-toxic to explants. Subjecting the sweet potato explants to sterilization for 12 and 15 min were found to be the most efficient, since there was no significant difference between them. These findings will help alleviate much of the burden associated with initiation of sweet potato cultures thereby reducing cost of plantlets production.

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How to cite this article:

R. Hammond, J. N. Buah, P.A. Asare and S. Acheampong, 2014. Optimizing Sterilization Condition for the Initiation of Sweet Potato (Ipomoea batatas) Culture in vitro. Asian Journal of Biotechnology, 6: 25-37.

DOI: 10.3923/ajbkr.2014.25.37

URL: https://scialert.net/abstract/?doi=ajbkr.2014.25.37

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