Effect of Low Temperature, Radiation and their Combination Treatments on Microorganisms Associated with Fresh Water Mola Fish, Amblypharyngodon mola (Hamilton-Buchanan 1822)

Research Article

**Effect of Low Temperature, Radiation and their Combination Treatments on Microorganisms Associated with Fresh Water Mola Fish, Amblypharyngodon mola (Hamilton-Buchanan 1822)**

Abdullah-Al- Mahin, Md. Golam Sorrowar, Md. Zahid Hasan, Md. Mansurul Haque and Harun-Or- Rashid

This study was conducted to assess microbiological quality of a local fresh water fish of Bangladesh named mola, Amblypharyngodon mola. The effects of freezing temperature, irradiation and their combination treatments on the associated microorganisms during storage of the fish were also evaluated. For this purpose, fish samples were collected from a local market and irradiated with different doses (2.5, 5.0, 7.5 and 10.0 kGy) of gamma radiation and kept at -20°C for 6 months followed by microbiological assay after each month interval. The average Total Viable Bacterial Count (TVBC), Total Staphylococcal Count (TSC), Total Coliform Count (TCC), Total Faecal Coliform Count (TFCC), Total Aeromonas Count (TAC) and Total Fungi Count (TFC) of fresh mola fish samples were 9.74x10⁰⁷, 3.60x10⁰⁶, 9.70x10⁰⁴, 6.40x10⁰⁵, 4.00x10⁰⁵ and 2.30x10⁰³cfu g^-1, respectively. Seventy six bacterial strains were isolated and identified from these samples which include Staphylococcus aureus, Micrococcus varians, Aeromonas hydrophila, Pseudomonas aeruginosa, Escherichia coli, Klebsiella ozaenae, Bacillus subtilis, Bacillus megaterium, Klebsiella edwardsii and Micrococcus radiodurans. When treated with gamma radiation dose, the total coliform, faecal coliform, Aeromonas sp. and fungi of the samples were eliminated at 2.5 kGy and total viable bacteria and staphylococci were eliminated at 7.5 kGy. During frozen storage, microbial counts were gradually decreased with time. However, the combination of these two treatments was found more effective than any of the single treatment for elimination of the associated microorganisms. The moisture contents of mola fish were not changed significantly at different stage of storage periods and also at different radiation doses. On the other hand, losses of total protein contents were also very low with increasing storage time and radiation dose. The findings indicated that, the combination treatment can be applied for long time preservation of mola fish in Bangladesh without any significant loss of moisture and protein values.

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Abdullah-Al- Mahin, Md. Golam Sorrowar, Md. Zahid Hasan, Md. Mansurul Haque and Harun-Or- Rashid, 2011. Effect of Low Temperature, Radiation and their Combination Treatments on Microorganisms Associated with Fresh Water Mola Fish, Amblypharyngodon mola (Hamilton-Buchanan 1822). Asian Journal of Biotechnology, 3: 507-518.

DOI: 10.3923/ajbkr.2011.507.518

URL: https://scialert.net/abstract/?doi=ajbkr.2011.507.518

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oti jemmimah Reply

27 October, 2011

pls more short note on ur work with references.

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Authors

Abdullah-Al- Mahin

Md. Golam Sorrowar

Md. Zahid Hasan

Md. Mansurul Haque

Harun-Or- Rashid

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Research Article

**Effect of Low Temperature, Radiation and their Combination Treatments on Microorganisms Associated with Fresh Water Mola Fish, Amblypharyngodon mola (Hamilton-Buchanan 1822)**

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