Assessment of Genetic Diversity and its Usefulness for Varietal Identification in Indian Elite Varieties of Wheat (T. aestivum L. em thell.) Using RAPD Markers


	Asian Journal of Biotechnology
	Year: 2011 \| Volume: 3 \| Issue: 5 \| Page No.: 460-469 DOI: 10.3923/ajbkr.2011.460.469

Assessment of Genetic Diversity and its Usefulness for Varietal Identification in Indian Elite Varieties of Wheat (T. aestivum L. em thell.) Using RAPD Markers

Reetesh Kumar, Sanjay K. Jaiswal, Manish K. Vishwakarma, Ved P. Rai, Priti Upadhyay and Arun K. Joshi

Abstract: Evaluation of genetic diversity was made using RAPD as a genetic marker system in allohexaploid wheat (T. aestivum). A set of 30 RAPD markers were used for 43 wheat cultivars to examine their utility for detecting DNA polymorphism in genotypes and estimating genetic diversity among them. A total of 228 different RAPD bands were scored for the presence of bands among all the varieties. Out of 30 oligonucleotide primers used, 5 primers gave reproducible DNA product (bands) suitable for the establishment of genetic diversity among the varieties. The Genetic Similarity (GS) coefficient for all pairs of genotypes was ranged from 0.66 to 1.0. V 35 (HD 2747) and V 39 (HI 8498) had high coefficient of similarity (0.982) while, V 4 (VL 804) and V 6 (VL 809) showed less coefficient of similarity (0.646). It indicated that V 35 (HD 2747) and V 39 (HI 8498) carry closely related ancestry while the cultivars V 4 (VL 804) and V 6 (VL 809) are not close to each other. Present results revealed the potential of RAPD technique in determining genetic diversity and make suitable fingerprints for wheat cultivars.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Asian Catfish Clarias batrachus (Linnaeus, 1758) Getting Critically Endangered

•	Assessment of Genetic Relationship among 15 Citrus Fruits Using RAPD

How to cite this article:

Reetesh Kumar, Sanjay K. Jaiswal, Manish K. Vishwakarma, Ved P. Rai, Priti Upadhyay and Arun K. Joshi, 2011. Assessment of Genetic Diversity and its Usefulness for Varietal Identification in Indian Elite Varieties of Wheat (T. aestivum L. em thell.) Using RAPD Markers. Asian Journal of Biotechnology, 3: 460-469.

DOI: 10.3923/ajbkr.2011.460.469

URL: https://scialert.net/abstract/?doi=ajbkr.2011.460.469

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar

Follow @scialert

Science Alert