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Asian Journal of Biotechnology

Year: 2011 | Volume: 3 | Issue: 4 | Page No.: 415-421
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Authors

Burcu Tuncer

Ruhsar Yanmaz

Keywords

Research Article

Induction of Microspore Embryogenesis in Ornamental Kale by Gamma Irradiation and High Temperature Stress

Burcu Tuncer and Ruhsar Yanmaz
The effect of gamma irradiation with high temperature treatments on the microspore embryogenesis were evaluated in ornamental kale (B. oleracea var. acephala cv. Chidori Red F1). Four different gamma doses (50, 75, 100 and 300 Gy) were applied to the buds that were at the late uninucleate stage microspore. Buds to which gamma applied were kept in 4°C under dry (in petri dish) and liquid (in NLN-13 medium) storage conditions. Microspores isolated after 29 h following the gamma treatment were taken and cultured in NLN-13 medium (40.000 microspore mL-1). In order to induce embryo induction, microspores were kept in 32 and 35°C during the first 2 days of the culture. The effect of the gamma irradiation treatment on ornamental kale varied by the temperature. According to the 12 day embryo yields after planting 32C°+50 Gy (5.54 embryo/petri) and 35°C + 75 Gy (5.20 embryo/petri) in ornamental kale were found effective treatments. The effect of keeping the irradiated buds under liquid storage conditions (in NLN-13 medium) up to isolation time was seen on providing embryo induction depending on temperature. As a result at both temperature treatment (32 and 35°C) on condition that microspores are isolated immediately after irradiation treatment intermediate doses below 100 Gy is thought to be more effective in stimulating the formation of the embryo.
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