Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Asian Journal of Biotechnology
  Year: 2011 | Volume: 3 | Issue: 3 | Page No.: 275-279
DOI: 10.3923/ajbkr.2011.275.279
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

An Efficient Method for Gene Disruption in Brucella abortus by Overlap Extension PCR

S. Nahid, M. Haghkhah, M. Saeb and M. Aminlari

Abstract:
An efficient method to perform gene disruption plasmid by overlap extension PCR for site directed mutagenesis in Brucella abortus is demonstrated in this study. This method is based on three standard PCR and one fusion PCR reactions for construction of gene disruption construct and then insertion into a vector for performing of gene disruption plasmid. The present method in despite of conventional methods, is not restriction site dependent and require only one step host cell cloning.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

S. Nahid, M. Haghkhah, M. Saeb and M. Aminlari, 2011. An Efficient Method for Gene Disruption in Brucella abortus by Overlap Extension PCR. Asian Journal of Biotechnology, 3: 275-279.

DOI: 10.3923/ajbkr.2011.275.279

URL: https://scialert.net/abstract/?doi=ajbkr.2011.275.279

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom