Short Communication

RAPD Fingerprinting and Demonstration of Genetic Variation in Three Pathogens Isolated from Mangrove Environment

M. Thangaraj, Vishruth Prem, T. Ramesh and A.P. Lipton

The aim of the study was to find the RAPD finger printing of Pseudomonas aeruginosa, Bacillus circulans and Serratia marcescens, using ten random primers. The RAPD-PCR produced reproducible electrophoretic band patterns only in seven primers. There were 67 species specific bands observed, among these, 25 bands in P. aeruginosa, 19 in B. circulans and 23 in S. marcescens. This study suggests that RAPD typing could be an additional rapid typing method for studying the epidemiology of these pathogens. Based on the RAPD data the genetic identity between S. marcescens and B. circulans was more (0.3803) than the other.

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M. Thangaraj, Vishruth Prem, T. Ramesh and A.P. Lipton, 2011. RAPD Fingerprinting and Demonstration of Genetic Variation in Three Pathogens Isolated from Mangrove Environment. Asian Journal of Biotechnology, 3: 269-274.

DOI: 10.3923/ajbkr.2011.269.274

URL: https://scialert.net/abstract/?doi=ajbkr.2011.269.274

Asian Journal of Biotechnology

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Authors

M. Thangaraj

Vishruth Prem

T. Ramesh

A.P. Lipton

Keywords

Short Communication

RAPD Fingerprinting and Demonstration of Genetic Variation in Three Pathogens Isolated from Mangrove Environment

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