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Asian Journal of Biotechnology
  Year: 2011 | Volume: 3 | Issue: 2 | Page No.: 194-199
DOI: 10.3923/ajbkr.2011.194.199
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In vitro Evaluation of Antibacterial Activities of Crude Extracts of Withania somnifera (Ashwagandha) to Bacterial Pathogens

Shanthy Sundaram, Priyanka Dwivedi and Shalini Purwar

The present aim of study is to detect the antibacterial property in the extracts of Withania somnifera. The antibacterial activity of Withania somnifera was tested on clinically isolated bacterial pathogens, i.e., Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis causing infections in human beings. Different solvents as ethanol, ethyl acetate, dichloromethane, hexane (from higher polarity to lower polarity) extracts was used for the study of antibacterial activity, by agar well diffusion method. The bacterial plates were prepared by nutrient agar. The extracts at different concentration from 10 to 40 mg mL-1 were loaded in the wells prepared in nutrient agar. Zone of inhibition was measured around the wells to check the antibacterial activity of extracts. Results showed the polar solvents to have higher antibacterial property in comparison to the nonpolar solvents. Relatively higher Minimum Inhibitory Concentration (MIC) were obtained for both gram positive bacteria S. aureus, B. subtilis and gram negative bacteria, E. coli and P. aeruginosa, with polar extract; however, less inhibitory effect was noted for nonpolar extracts. Ethyl acetate extract possesses great inhibitory activity for gram positive bacteria, S. aureus followed by B. subtilis. Among gram negative bacteria, highest inhibitory effect was observed with P. aeruginosa followed by E. coli. Antimicrobial activity of crude extract of W. somnifera were carried out to validate the use of traditional medicinal herbal and the results of this study tend to give credence to the common use of W. somnifera plant.
PDF Fulltext XML References Citation Report Citation
  •    Evidence Based Antibacterial Potentials of Medicinal Plants and Herbs Countering Bacterial Pathogens Especially in the Era of Emerging Drug Resistance: An Integrated Update
  •    Ashwagandha (Withania somnifera): Role in Safeguarding Health, Immunomodulatory Effects, Combating Infections and Therapeutic Applications: A Review
  •    Antimicrobial Activity of Cinnamate-eugenol: Synergistic Potential, Evidence of Efflux Pumps and Amino Acid Effects
  •    Antibacterial and Antioxidative Activities of the Various Solvent Extracts of Banana (Musa paradisiaca cv. Mysore) Inflorescences
  •    Biochemical Composition of Roots of Withania somnifera (L.) Dunal
  •    Antibacterial Activity of Extracts of Piper longum
How to cite this article:

Shanthy Sundaram, Priyanka Dwivedi and Shalini Purwar, 2011. In vitro Evaluation of Antibacterial Activities of Crude Extracts of Withania somnifera (Ashwagandha) to Bacterial Pathogens. Asian Journal of Biotechnology, 3: 194-199.

DOI: 10.3923/ajbkr.2011.194.199






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