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Asian Journal of Biotechnology
  Year: 2010 | Volume: 2 | Issue: 2 | Page No.: 139-145
DOI: 10.3923/ajbkr.2010.139.145
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Screening of Local Actinomycete Isolates in Manipur for Anticandidal Activity

Suchitra Sanasam and Debananda S. Ningthoujam

The present study was aimed at screening of local actinomycete isolates for anticandidal activity, selection and characterization of potent strain (s) and initial optimization of fermentation conditions for anticandidal production. Of 35 actinomycete isolates in our collection from various biotopes in Manipur that were screened by cross-streak method, 2 strains (5.7% of isolates screened) NRP1-14 and NRP1-26, obtained from Nambul river in Imphal, Manipur, India, showed anticandidal activity. Based on relative potency, NRP1-14 was selected for further studies. It has been identified as Streptomyces mutabilis NRP1-14 based on phenotypic and genotypic characteristics. Among various media tested for submerged fermentation of the bioactive strain, Starch Casein Nitrate (SCN) broth was found to be the best. The strain produced significant activity against Candida albicans MTCC 227 in SCN broth after 3 days of incubation in SCN broth of pH 7.2 at 28°C under shaking speed of 150 rpm. The NRP1-14, interestingly, also exhibited significant antagonistic activity against several rice fungal pathogens-Bipolaris oryzae, Curvularia oryzae, Fusarium oxysporum and Pyricularia oryzae. Anticandidal activity is being optimized by improving cultural and environmental conditions for fermentation. This study shows that biotopes in Manipur can yield promising actinomycetes for use in medicine and agriculture. To our knowledge, this is the first report of a Streptomyces mutabilis strain with potent anticandidal activity. The NRP1-14, therefore, warrants further investigation for possible application as anticandidal and biocontrol agents (BCAs).
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  •    Taxonomy and Antimicrobial Activity of Streptomyces coeruleorubidus sp. Isolated from Marine Sediment
  •    Studies on Actinomycetes Diversity in Eastern Ghats (Yercaud Hills) of Southern India for Secondary Metabolite Production
How to cite this article:

Suchitra Sanasam and Debananda S. Ningthoujam, 2010. Screening of Local Actinomycete Isolates in Manipur for Anticandidal Activity. Asian Journal of Biotechnology, 2: 139-145.

DOI: 10.3923/ajbkr.2010.139.145






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