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Asian Journal of Biotechnology
  Year: 2009 | Volume: 1 | Issue: 2 | Page No.: 67-73
DOI: 10.3923/ajbkr.2009.67.73
Potentiometric Zn2+ Biosensor Based on Bacterial Cells
M. Datta, S. Mittal and D. Goyal

Pseudomonas striata cell mass was immobilized in polyvinylchloride (PVC), a neutral carrier to prepare zinc selective membranes using Dibutyl-phthalate as the plasticizer. Membranes were prepared using 1, 2, 3, 5 and 7% of bacterial biomass, of which the highest response was obtained for 5%. Potentiometric response of the electrode was studied for Zn, Cd and Cu in the concentration range of 10-7 to 10-1 M. A linear trend between the electrode response and the varying metal concentrations was seen only for Zn2+ ions in the range of 10-4 to 10-1 M. Calibration slope of 22 mV/decade and detection limit of 510-4 M was obtained for zinc. Electrode showed a sharp response time of 6 sec and pH optima of 3. Thus, the PVC membrane containing the bacterial biomass was found to selectively bind the Zn2+ ions and generate the corresponding potential response at different zinc concentrations.
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How to cite this article:

M. Datta, S. Mittal and D. Goyal, 2009. Potentiometric Zn2+ Biosensor Based on Bacterial Cells. Asian Journal of Biotechnology, 1: 67-73.

DOI: 10.3923/ajbkr.2009.67.73