Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Asian Journal of Biotechnology
  Year: 2009 | Volume: 1 | Issue: 1 | Page No.: 37-41
DOI: 10.3923/ajbkr.2009.37.41
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Use of Molecular Marker for Assay Gene Dosage Resistant Gene to Rhizomania Disease (Rz1) in Sugar beet (Beta vulgaris L.)
Nouhi Ali Askar, Amiri Reza, Hagh Nazari Ali, Saba Jalal and Mesbah Mahmood

Abstract:
This study verified the effect of infected soil on resistant plants varieties to rhizomania disease and with using a molecular marker discriminated homozygote from heterozygote genotypes which this theory performed with use of infected soil that was provided from infected fields then greenhouse test was done to identify resistant and susceptible plants. Extracted DNA from leaves of resistant and susceptible plants was bulked to provide two bulks for resistant and susceptible plants then verified affect of allele (R) on increase resistance to Rhizomania disease. The F2 population for study obtained from cross between Holly1-4 as resistant parent and annual cultivar as susceptible parent. In this study three-hundred RAPD primers was used for detection of molecular markers linked to resistance gene. Finally the gene (Rz1) was tagged with using of two RAPD primers and one of the markers was OP-AN9 which was mapped 13.7 cM apart from Rz1 gene in repulsion phase. Then with t-test statistical method and repulsion marker verified equal of variations related to two population heterozygote and dominant homozygote genotypes. Finally accepted hypothesis equal of variation between two population at p≤0.05. This result show don’t effecting additional one allele (R) to resistance again Rhizomania disease.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Nouhi Ali Askar, Amiri Reza, Hagh Nazari Ali, Saba Jalal and Mesbah Mahmood, 2009. Use of Molecular Marker for Assay Gene Dosage Resistant Gene to Rhizomania Disease (Rz1) in Sugar beet (Beta vulgaris L.). Asian Journal of Biotechnology, 1: 37-41.

DOI: 10.3923/ajbkr.2009.37.41

URL: https://scialert.net/abstract/?doi=ajbkr.2009.37.41

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

       

       

Curve Bottom