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Asian Journal of Biochemistry
  Year: 2014 | Volume: 9 | Issue: 1 | Page No.: 41-48
DOI: 10.3923/ajb.2014.41.48
 
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Correlation Studies on Human Seminal Plasma Proteins and Their Relation with Semen Freezablity
A.S. Vickram, V. Devi Rajeswari, M. Srinivas, G. Jayaraman, R.A. Kamini, M. Ramesh Pathy, S. Ventat Kumar and T.B. Sridharan

Abstract:
Purpose of this research was to elucidate the protein profiles of the seminal plasma in various categories of male infertility, to scrutinize their correlation with seminal parameters. Oligoasthenospermia (N = 15), asthenospermaia (N = 17), azoospermia (N = 12), normospermia (N = 27), oligospermia (N = 12) and fertile (control subjects, N = 10) were collected. The samples were diluted by tris-egg yolk extender and were frozen. Plasma was separated from semen by centrifugation, underwent SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE). The mean values with its standard error of semen parameters of fresh sample were shown significant difference (p<0.0001) when compared to the post-thaw samples. Of the various fractionations, the protein with molecular weight 44.6 kDa shows high significant and positive correlation (p<0.01) with sperm concentration of freshly evaluated semen samples and low level significant (p<0.00001) with the frozen samples. Sperm motility was positively correlated (p<0.029) with the protein molecular weight 56.6 kDa in the freeze thawed semen samples. This reality could sustain the implication that seminal plasma proteins act on the sperm physiology and morphology and it found to act on strange ways. Supplementary studies are essential to define the mechanism of different proteins involved in the fertilization and their correlation.
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How to cite this article:

A.S. Vickram, V. Devi Rajeswari, M. Srinivas, G. Jayaraman, R.A. Kamini, M. Ramesh Pathy, S. Ventat Kumar and T.B. Sridharan, 2014. Correlation Studies on Human Seminal Plasma Proteins and Their Relation with Semen Freezablity. Asian Journal of Biochemistry, 9: 41-48.

DOI: 10.3923/ajb.2014.41.48

URL: https://scialert.net/abstract/?doi=ajb.2014.41.48

 
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