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Asian Journal of Biochemistry
  Year: 2014 | Volume: 9 | Issue: 3 | Page No.: 142-150
DOI: 10.3923/ajb.2014.142.150
Discrimination of Codon 72 p53 Polymorphisms using High Resolution Melting Curve Method
Mohammad Abdi, Bayazid Ghaderi, Sabrieh Amini, Arghavan Baban, Sonya Najafpour, Sahar Naderi and Shamisa Pourdad

Abstract:
One of the most important polymorphism in p53 gene occurs in codon 73 of exon 4. Individuals who have Arg/Arg allele may have an increase risk for progress of cancer. The present study was aimed to develop a rapid, inexpensive and simple high resolution melting curve analysis method to distinguish different genotype of codon 72 p53. This study was performed on 167 patients with four common cancers in Kurdistan. High resolution melt analysis of PCR products revealed different allele of codon 72 p53 gene. Genotyping by high resolution melt analysis was verified by DNA sequencing. The samples were categorized as Arg/Arg (31%), Arg/Pro (44%) and Pro/Pro (25%) in four cancer types. Patients with colorectal cancer had the highest frequency of Arg/Arg polymorphism (65%). High resolution melting analysis was completely compatible with sequencing results. The results indicate individuals with Arg/Arg allele of codon 72 p53 gene have an increased risk for development of cancer in Kurd ethnicity. In addition high resolution melting analysis is useful for rapid differentiation of p53 alleles and can be used for diagnostic purposes directly from clinical samples, as well as epidemiological studies.
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How to cite this article:

Mohammad Abdi, Bayazid Ghaderi, Sabrieh Amini, Arghavan Baban, Sonya Najafpour, Sahar Naderi and Shamisa Pourdad, 2014. Discrimination of Codon 72 p53 Polymorphisms using High Resolution Melting Curve Method. Asian Journal of Biochemistry, 9: 142-150.

DOI: 10.3923/ajb.2014.142.150

URL: https://scialert.net/abstract/?doi=ajb.2014.142.150

 
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