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Asian Journal of Biochemistry
  Year: 2011 | Volume: 6 | Issue: 1 | Page No.: 98-103
DOI: 10.3923/ajb.2011.98.103
 
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Methaemoglobin Content and NADH-methaemoglobin Reductase Activity of Three Human Erythrocyte Genotypes

P.C. Chikezie

Abstract:
The aim of the present study was to ascertain methaemoglobin concentrations and levels of NADH-methaemoglobin reductase activity of three human erythrocyte genotypes, namely, HbAA, HbAS and HbSS. The cyanomethaemoglobin reaction was used for the determination of erythrocyte haemolysate methaemoglobin concentration. NADH-methaemoglobin reductase activity was measured by the rate of oxidation of NADH + H+ when, the erythrocyte enzyme was incubated in potassium ferrocyanide {K3Fe(CN)6}. Whereas, methaemoglobin concentrations in the three erythrocyte genotypes was in the range of 1.45±0.13 to 2.50±0.43%, in the order: HbAS<HbAA<HbSS, levels of NADH-methaemoglobin reductase activity ranged between 8.86±2.49 and 14.77±1.47 IU gHb-1, in the order of: HbSS<HbAS<HbAA. However, there was no significant difference (p<0.05) in methaemoglobin concentration and NADH-methaemoglobin reductase activity between HbAA and HbAS erythrocytes. The results showed a relationship between erythrocyte NADH-methaemoglobin reductase activity and methaemoglobin concentration.
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How to cite this article:

P.C. Chikezie , 2011. Methaemoglobin Content and NADH-methaemoglobin Reductase Activity of Three Human Erythrocyte Genotypes. Asian Journal of Biochemistry, 6: 98-103.

DOI: 10.3923/ajb.2011.98.103

URL: https://scialert.net/abstract/?doi=ajb.2011.98.103

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