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Asian Journal of Biochemistry
  Year: 2011 | Volume: 6 | Issue: 4 | Page No.: 322-336
DOI: 10.3923/ajb.2011.322.336
Molecular Classification of Members in LMW Glutenin Family of Wild Emmer Wheat (Triticum dicoccoides, AABB)
Li-Juan Yang, Ya-Xi Liu, Bi-Ling Xu, Wei Li and Guo-Yue Chen

Abstract:
One hundred and thirteen low-molecular-weight glutenin subunits encoding sequences and parts of upstream were characterized from Triticum dicoccoides. The encoded proteins of 113 genes had similar structures to previously characterized LMW-GS. These sequences had 856~1402 nucleotides in length with 2~26 repeat motifs. Most of the sequences were typical LMW-m glutenin subunits genes and the frequency of SNPs was 1.6 out of 10 bases and A-G mutation was the most frequent. Fourteen deduced amino acid sequences were found to be possessed with an additional cysteine residue in C-ter I. Thirty-six haplotypes were detected and phylogenetic analysis indicated that the 36 haplotypes could be classified into 3 haplotype groups. Individually classifications based on the four main domains of LMW-GS DNA sequences, 5’flanking, single peptide, N-terminals and C-terminal, were in agreement with the classification based on the coding regions. Consilient evolution was found between domains of LMW-GS as well as each domain and the whole coding region. The results revealed the important information of low-molecular-weight glutenin subunit gene family and contributed to our understanding of functional aspects of the low-molecular-weight glutenin subunit genes.
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How to cite this article:

Li-Juan Yang, Ya-Xi Liu, Bi-Ling Xu, Wei Li and Guo-Yue Chen, 2011. Molecular Classification of Members in LMW Glutenin Family of Wild Emmer Wheat (Triticum dicoccoides, AABB). Asian Journal of Biochemistry, 6: 322-336.

DOI: 10.3923/ajb.2011.322.336

URL: https://scialert.net/abstract/?doi=ajb.2011.322.336

 
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