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Asian Journal of Biochemistry
  Year: 2008 | Volume: 3 | Issue: 3 | Page No.: 169-175
DOI: 10.3923/ajb.2008.169.175
Immobilization of α-Amylase from Acha (Digiteria exilis) on Different Cellulose Fibre Materials
E.C. Egwim and O.B. Oloyede

Abstract:
Alpha amylase was obtained from Acha (D. exilis) sprouted for 96 h and immobilized on palm wood chips, coconut and cotton wood fibers according to standard procedures. Palm wood chips maintained enzyme activity up to 25 h and still retained about 7x10-2 mg glucose mL min-1 of residual activity while coconut and cotton wool lost all activity by 12 h. Enzyme half-life improved by 857.0, 21.4 and 28.6 folds using palm wood chips, coconut and cotton wool fibre as carriers, respectively. Palm wood clip improved Vmax and catalytic efficiency by 177 and 163%, respectively. The results concludes that palm wood chip can be used to immobilize α-amylase from D. exilis for several cycles of reuse.
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How to cite this article:

E.C. Egwim and O.B. Oloyede, 2008. Immobilization of α-Amylase from Acha (Digiteria exilis) on Different Cellulose Fibre Materials. Asian Journal of Biochemistry, 3: 169-175.

DOI: 10.3923/ajb.2008.169.175

URL: https://scialert.net/abstract/?doi=ajb.2008.169.175

 
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