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Asian Journal of Biochemistry
  Year: 2007 | Volume: 2 | Issue: 1 | Page No.: 31-41
DOI: 10.3923/ajb.2007.31.41
Treatment of Streptozotocine Induced Diabetes Mellitus in Male Rats by Immunoisolated Transplantation of Purified Langerhans Islet Cells
A. Akbarzadeh, D. Noruzian, SH. Jamshidi, A. Farhangi, M.R. Mehrabi, B. Lame Rad, M. Mofidian and A. Allahverdi

Abstract:
Induction of experimental diabetes mellitus is indeed, the first step in the process of purification of pancreatic Langerhans islet cells of normal rats for transplantation under the testis subcutaneous of experimentally induced diabetic rats. For induction of experimental diabetes in male adult rats weighting 250-300 g (75-90 days), 60 mg kg-1 of streptozotocin was injected intravenously. Biopsy of pancreas tissue of diabetic and normal rats showed that the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In the process of purification of islet cells, after collagenase digestion of pancreases, islets were isolated and dissociated; employing enzymes like DNase and trypsine, so the islet cells were changed into single cells and these cells were assayed by flow cytometry. Flow cytometry of these cells indicated that there were 91% of beta cells in cell suspension. Donor tissue in each step of this research was prepared from 38 adult wistar male rats. Transplantation was done in rats after 2-4 weeks induction of diabetes. Encapsulation of pancreatic islet cells allows for transplantation in the absence of immunosuppression. This technique which is called immunoisolation is based on the principle that transplanted tissue is protected for the host immune system by an artificial or natural membrane. The diabetic, treated and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured. Analysis of variance showed a high significant difference between them.
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How to cite this article:

A. Akbarzadeh, D. Noruzian, SH. Jamshidi, A. Farhangi, M.R. Mehrabi, B. Lame Rad, M. Mofidian and A. Allahverdi, 2007. Treatment of Streptozotocine Induced Diabetes Mellitus in Male Rats by Immunoisolated Transplantation of Purified Langerhans Islet Cells . Asian Journal of Biochemistry, 2: 31-41.

DOI: 10.3923/ajb.2007.31.41

URL: https://scialert.net/abstract/?doi=ajb.2007.31.41

 
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