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Asian Journal of Biochemistry
  Year: 2006 | Volume: 1 | Issue: 2 | Page No.: 169-177
DOI: 10.3923/ajb.2006.169.177
Comparative Studies on the Ability of Crude Polyphenols from Some Nigerian Citrus Peels to Prevent Lipid Peroxidation-In vitro
G. Oboh and A.O. Ademosun

Abstract:
This study sought to determine the potential of citrus peels as a functional powder to protect lipid peroxidation in animal tissues-in vitro. Polyphenols were extracted from sundried peels of orange, grape, shaddock and tangerine using 80% Acetone; the total phenol, ferric reducing antioxidant capacity and the ability of the extract to prevent lipid peroxidation in some isolated organs in cow (liver, intestine, brain, heart and kidney) were determined using TBARS (Thiobarbituric reactive species). The peels had high total phenol (2.9-3.2 mg g 1) content and reducing power (0.52-0.76 OD700), however peels from orange had the highest total phenol content (3.2 mg g 1), while peels from tangerine had the highest reducing power (0.76 OD700). The polyphenols extract from all the citrus peels at the concentration tested (6.0-33.3 µg mL 1) caused a marked decrease in the lipid peroxidation in the various organs tested. In the presence of 33.3 µg mL 1 of the extract, there were 8.0 (Orange)-50% (Shaddock) lipid peroxidation in the Cow’s brain, 78 (Tangerine)-87% (Orange) in the liver, 30% (Orange)-50% (Shaddock) in intestine, 40 (Orange)-66% (Grape) in kidney and 66 (Tangerine)-89% (Grape) in the heart when compared to the untreated organ (100%). The polyphenols extract from Orange and Tangerine peels appear to protect the various organs tested better than that of Shaddock and Grape. Citrus peels powder could therefore be use as a cheap natural antioxidant in food preservation and as a source of dietary antioxidant supplement in human and livestock feed.
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How to cite this article:

G. Oboh and A.O. Ademosun , 2006. Comparative Studies on the Ability of Crude Polyphenols from Some Nigerian Citrus Peels to Prevent Lipid Peroxidation-In vitro. Asian Journal of Biochemistry, 1: 169-177.

DOI: 10.3923/ajb.2006.169.177

URL: https://scialert.net/abstract/?doi=ajb.2006.169.177

 
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