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Asian Journal of Animal and Veterinary Advances

Year: 2017 | Volume: 12 | Issue: 4 | Page No.: 189-196
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Authors

Sudu Hakuruge Madusha Pramud Wimalasena

Gee-Wook Shin

Hansani Nilupama Kumari Senarath Pathirana

Benthotage Chamara Jayasankha De Silva

Sabrina Hossain

Gang-Joon Heo

Gang-Joon Heo

Keywords

Background and Objective: Drug resistance in bacteria is a challenge both in human and veterinary medicine. This study was conducted to characterize quinolone resistant determinants in Morganella morganii isolated from pet turtles. Materials and Methods: Antimicrobial susceptibility of twenty-two M. morganii isolates against nalidixic acid, ciprofloxacin, ofloxacin and levofloxacin was examined by disk diffusion assay and the Minimum Inhibitory Concentration (MIC). Substitutions of the Quinolone Resistance Determining Region (QRDR) and Plasmid Mediated Quinolone Resistance (PMQR) genes were detected using conventional PCR assays and sequencing. Results: Three isolates were resistant to the all tested quinolones and one isolate was resistant only to nalidixic acid. In QRDR substitution analysis, three isolates displayed the Ser463Ala, Ser464Tyr and novel Glu466Asp substitutions in gyrB and the Ser80Ile substitution in parC. Two isolates displayed only Ser463Ala substitution in gyrB. The unique PMQR gene detected was qnrD, which was found in 59% of the isolates. The aac-(6’)-Ib-cr gene variant was identified in 50% of the isolates. In addition, neighbor-joining phylogenetic tree derived using gyrB gene sequences exhibited two distinct clads comprising, first; present study isolates with a quinolone-resistant isolate of human clinical origin and second; isolates of environmental origin. Conclusions: All results suggest healthy pet turtles might serve as a potential reservoir for quinolone-resistant M. morganii due to the high prevalence of PMQR determinants, especially, qnrD and target gene alterations in QRDR together with a novel mutation in gyrB.
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Sudu Hakuruge Madusha Pramud Wimalasena, Gee-Wook Shin, Hansani Nilupama Kumari Senarath Pathirana, Benthotage Chamara Jayasankha De Silva, Sabrina Hossain and Gang-Joon Heo, 2017. Characterization of Quinolone Resistant Determinants in Morganella morganii Isolated from Pet Turtles. Asian Journal of Animal and Veterinary Advances, 12: 189-196.

DOI: 10.3923/ajava.2017.189.196

URL: https://scialert.net/abstract/?doi=ajava.2017.189.196

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