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Asian Journal of Animal and Veterinary Advances

Year: 2017 | Volume: 12 | Issue: 1 | Page No.: 17-23
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Authors

Gulten Ates

Elif Ozkok

Hatice Yorulmaz

Irfan Aydin

Sule Tamer

Keywords

Research Article

Effects of Ghrelin on Inflammation and Oxidative Stress Parameters in Sepsis-induced Liver Tissue of Rat

Gulten Ates, Elif Ozkok, Hatice Yorulmaz, Irfan Aydin and Sule Tamer
Background: Sepsis is a life-threatening systemic inflammatory syndrome, that affects many organs or systems. Ghrelin is released in many tissues and organs and it has anti-inflammatory and antioxidant effects. Objective: In this study, the effects of exogenous ghrelin on Tumor Necrosis Factor-α (TNF-α), interleukin-10 (IL-10), superoxide dismutase (SOD), thiobarbituric acid reactive substances (TBARS) and ghrelin levels were aimed to investigate in liver tissue after inducing sepsis with lipopolysaccharide (LPS). Materials and Methods: Wistar albino rats were divided into four groups, control, LPS, ghrelin, ghrelin+LPS. The LPS group was established by giving LPS twice (5 mg kg–1, i.v. and i.p., respectively). Ghrelin (10 nmol kg–1) was given intravenously. Tissue levels of TNF-α, IL-10, ghrelin and the activities of SOD were mesured with using an ELISA kit. The levels of TBARS were determined spectrophotometrically. Results: The levels of TNF-α and IL-10 were found higher in the LPS group than controls (p<0.01 and p<0.05, respectively) and TBARS levels were increased in the LPS group compared with the controls, ghrelin and ghrelin+LPS groups (p<0.01, p<0.05 and p<0.01, respectively). The SOD and ghrelin levels were higher in the LPS group than in the others, ghrelin levels were also increased in the ghrelin group compared with the controls (p<0.05). According to histologic imaging, there were enlargements in sinusoids in the LPS group. In the ghrelin+LPS group, the same image was seen as in the controls. Conclusion: Ghrelin treatment attenuated anti- and pro-inflammatory cytokines, TBARS levels and SOD activities and also reduced liver tissue damage in a time and dose dependent manner during sepsis.
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