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Asian Journal of Animal and Veterinary Advances

Year: 2016 | Volume: 11 | Issue: 10 | Page No.: 608-619
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Authors

E.M.M. Abdel-Gawad

B.R. Abdel-Halim

N.A. Helmy

A.F. Badr

Keywords

Research Article

Effect of Cryoprotective Solutions, Ethylene Glycol, Dimethyle-sulfoxide and Ficoll 70 with Different Combination Ratios on Vitrification of Bovine Oocytes and Embryos Produced in vitro

E.M.M. Abdel-Gawad, B.R. Abdel-Halim, N.A. Helmy and A.F. Badr
Objective: The objective of the present study was to compare cryoprotective solutions such as Ethylene Glycol (EG), dimethyle-sulfoxide (DMSO) and ficoll 70 with different combination ratios for vitrification of mature bovine oocytes and embryos produced in vitro. In addition to the demonstration of the effect of the straw diameter on post thawing viability of the thawed matured oocytes and embryos. Materials and Methods: A total of 509 oocytes were collected from 175 ovaries by slicing technique. Matured oocytes frozen in solutions containing 20% EG+20% DMSO+0.3 M trehalose had mean survival rate of (44.43±4.98%). Mature oocytes frozen in solutions containing 40% EG and 18% ficoll 70 by a ratio of 1:1, 2:1 and 3:2 in volume had a mean survival rate of 49.22±1.66, 54.33±3.11 and 62.00±3.71%, respectively. Results: The present study revealed that blastocysts cryopreserved in media containing EG+ficoll (3:2) had a significantly (p<0.01) higher recovery rate (79.28±13.08) compared to 45.00±16.24 blastocyst in embryos cryopreserved in DMSO, respectively. Moreover, recovery rates of blastocysts cryopreserved in media containing EG+ficoll (2:1) and in media containing EG+ficoll (3:1) were numerically higher than those cryopreserved in DMSO group (50.00±3.74 and 63.49±6.83, respectively). Also using of ministraw for the cryopreservation of mature bovine oocytes had post-thawing viability significantly higher (<10%) than using midistraw. Conclusion: By this study it can be concluded that using of ministraw for the cryopreservation of mature bovine oocytes had post-thawing viability significantly higher (<10%) than using midistraw. So, combination of 40% EG+18% ficoll 70 by the ratio of 3:2 act as a good cryoprotectant combination for vitrification of mature bovine oocytes.
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How to cite this article

E.M.M. Abdel-Gawad, B.R. Abdel-Halim, N.A. Helmy and A.F. Badr, 2016. Effect of Cryoprotective Solutions, Ethylene Glycol, Dimethyle-sulfoxide and Ficoll 70 with Different Combination Ratios on Vitrification of Bovine Oocytes and Embryos Produced in vitro. Asian Journal of Animal and Veterinary Advances, 11: 608-619.

DOI: 10.3923/ajava.2016.608.619

URL: https://scialert.net/abstract/?doi=ajava.2016.608.619

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