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Development of New Indigenous Dot-ELISA Kit as Sensitive Field Based Herd Screening Test for the Diagnosis of Johnes Disease in the Domestic Buffalo Population |
Shoor Vir Singh ,
Sachin Digambar Audarya,
Manju Singh,
Bjorn John Stephen,
Daljeet Chhabra,
Kundan Kumar Chaubey,
Saurabh Gupta ,
Sahzad,
Anjali Pachoori,
Sujata Jayaraman,
Gajendra Kumar Aseri,
Jagdip Singh Sohal ,
Ashok Kumar Bhatia and Kuldeep Dhama |
Abstract:
Johnes disease is endemic in the domestic riverine buffalo population of the country and bio-load of Mycobacterium avium subspecies paratuberculosis is increasing in the absence of indigenous diagnostic kits and control programs. A new dot-ELISA kit has been developed and validated with indigenous plate ELISA for the screening of buffaloes against Johnes disease. Out of 156 serum samples screened 41.0 (64), 85.8 (134) and 85.2% (133) were positive for MAP infection by indigenous plate ELISA kit condition (A), condition (B) and indigenous dot ELISA, respectively. Dot-ELISA kit detected 85.2 (133) and 90.3% (141) buffaloes as positive together with indigenous plate ELISA kit in condition A and B, respectively. Comparison of Indigenous plate-ELISA with Indigenous dot-ELISA revealed substantial agreement between two tests. Study showed that Indigenous dot-ELISA test has potential to be sensitive and cost effective Field based herd screening test for the large scale screening of the domestic livestock population against Johnes disease. The study also showed that despite high slaughter rate, incidence of Johnes disease was high in native population of riverine buffaloes (Bubalus bubalis) and call for immediate control of disease.
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How to cite this article:
Shoor Vir Singh, Sachin Digambar Audarya, Manju Singh, Bjorn John Stephen, Daljeet Chhabra, Kundan Kumar Chaubey, Saurabh Gupta, Sahzad, Anjali Pachoori, Sujata Jayaraman, Gajendra Kumar Aseri, Jagdip Singh Sohal, Ashok Kumar Bhatia and Kuldeep Dhama, 2016. Development of New Indigenous Dot-ELISA Kit as Sensitive Field Based Herd Screening Test for the Diagnosis of Johnes Disease in the Domestic Buffalo Population. Asian Journal of Animal and Veterinary Advances, 11: 44-52.
DOI: 10.3923/ajava.2016.44.52
URL: https://scialert.net/abstract/?doi=ajava.2016.44.52
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