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Asian Journal of Animal and Veterinary Advances
  Year: 2016 | Volume: 11 | Issue: 3 | Page No.: 198-203
DOI: 10.3923/ajava.2016.198.203
 
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Osteogenic Effect of Percutaneous Implantation of Mononuclear Cell Fraction in Horses with Experimental Bone Fracture
Luiz Fernando de Oliveira Varanda, Julia de Miranda Moraes, Ana Lourdes Arrais de Alencar Mota, Andre Rodrigues da Cunha Barreto-Vianna, Kaique Nogueira, Roberta Ferro de Godoy and Eduardo Mauricio Mendes de Lima

Abstract:
Slow speed and poor quality of bone healing are some of the major obstacles faced by equine veterinary practitioners. Cellular therapies that may assist in the osteogenesis process have been evaluated experimentally and clinically in several animal species and humans. The study evaluated the osteogenic effect of autologous percutaneous implantation of Mononuclear Cell Fraction (MCF) of the Bone Marrow (BM) on the healing process of experimentally induced fracture in the IV metacarpal bone by determining the Bone Mineral Density (BMD) in five horses of both sexes. Five days after experimentally inducing the bone fracture, the BM was aspirated from the sternum of each horse and the MCF was isolated. Subsequently, each horse was subjected to the percutaneous implantation of the MCF with DMEM in the right forelimb (treated limb-TL) and only DMEM as control, in the left forelimb (control limb-CL). The BMD values were higher in the TL compared to the CL in six of the eleven time intervals at which the bone density was measured. The results showed that MCF stimulated osteogenesis by accelerating the bone healing process and increasing the amount of mineralized bone matrix. The percutaneous implantation was found to be a minimally invasive low-cost technique that can replace sophisticated and labour intensive methods of stem cell transplantation.
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How to cite this article:

Luiz Fernando de Oliveira Varanda, Julia de Miranda Moraes, Ana Lourdes Arrais de Alencar Mota, Andre Rodrigues da Cunha Barreto-Vianna, Kaique Nogueira, Roberta Ferro de Godoy and Eduardo Mauricio Mendes de Lima, 2016. Osteogenic Effect of Percutaneous Implantation of Mononuclear Cell Fraction in Horses with Experimental Bone Fracture. Asian Journal of Animal and Veterinary Advances, 11: 198-203.

DOI: 10.3923/ajava.2016.198.203

URL: https://scialert.net/abstract/?doi=ajava.2016.198.203

 
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