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Asian Journal of Animal and Veterinary Advances

Year: 2014 | Volume: 9 | Issue: 10 | Page No.: 621-640
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Authors

M. Saminathan

R.B. Rai

K. Dhama

G.J. Ranganath

V. Murugesan

K. Kannan

S. Pavulraj

A. Gopalakrishnan

C. Suresh

Keywords

Mammary tumours rank second as the most common neoplasms in dogs after skin tumours, whereas in women the most common cause of cancer-related deaths is breast cancer. N-Methyl-N-Nitrosourea (NMU) is a highly specific mammary gland carcinogen which directly act and does not require metabolic activation. In the present study, NMU at the dose rate of 50 mg kg-1 body weight was used intra-peritoneally for the induction of mammary tumour. The first palpable tumour appeared on 70th day post carcinogen injection and subsequently, most of the tumours were developed around 18-20th week. During 28 weeks of experimental period, the tumour incidence was 82.86% (29/35). The tumour frequency was found to be 4.7±0.33 tumours and the average latency period was 107±4.1 days. The average tumour volume was found to be 69±8.8 cm3. Equally, 50% of mammary tumours appeared on the right (22/44) and left (22/44) mammary gland chain. Region wise, 81.82% (36/44) of the tumours appeared on abdominal-inguinal mammary glands and 18.18% (8/44) on the cervical thoracic mammary glands. A total of 44 mammary tumours were diagnosed in which 88.64% (39/44) were malignant and 11.36% (5/44) were benign. Among the malignant tumours, 33.33% (13/39) were non-invasive and 66.67% (26/39) were invasive. The average values of mitotic index, Proliferating Cell Nuclear Antigen (PCNA), Vascular Endothelial Growth Factor (VEGF) and Platelet Endothelial Cell Adhesion Molecule (PECAM-1) in NMU induced mammary tumours were found to be 4.5±0.46/hpf, 77±2.6, 16.2±0.86 and 15±0.69, respectively. The present study for the first time demonstrated the expression of VEGF and PECAM-1/CD-31 proteins in NMU induced mammary tumours.
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How to cite this article

M. Saminathan, R.B. Rai, K. Dhama, G.J. Ranganath, V. Murugesan, K. Kannan, S. Pavulraj, A. Gopalakrishnan and C. Suresh, 2014. Histopathology and Immunohistochemical Expression of N-Methyl-N-Nitrosourea (NMU) Induced Mammary Tumours in Sprague-Dawley Rats. Asian Journal of Animal and Veterinary Advances, 9: 621-640.

DOI: 10.3923/ajava.2014.621.640

URL: https://scialert.net/abstract/?doi=ajava.2014.621.640

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