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Asian Journal of Animal and Veterinary Advances
  Year: 2014 | Volume: 9 | Issue: 4 | Page No.: 262-269
DOI: 10.3923/ajava.2014.262.269
 
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Serological and Molecular Detection of Brucella abortus from Cattle by RBPT, STAT and PCR and Sample Suitability of Whole Blood for PCR

K. Karthik, R. Rathore, P. Thomas, A. Elamurugan, T.R. Arun and K. Dhama

Abstract:
Brucella abortus, one of the major pathogen causing abortions in cattle worldwide and a zoonotic agent, need to be detected earlier in order to prevent its spread among animals. The present study aimed at to know the prevalence of B. abortus in cattle population of three states (Uttar Pradesh, Uttarakhand and Tamil Nadu) of India by serological (Rose Bengal Plate Test (RBPT) and Serum Tube Agglutination Test (STAT)) and molecular (polymerase chain reaction) detection in sera samples and whole blood (n = 370), respectively. Out of a total of 370 sera samples, 61 (16.49%) were positive by RBPT and 59 (15.94%) by STAT. Screening of the whole blood samples by genus specific bcsp31 gene based PCR as well as species specific IS711 gene based PCR revealed that 56 (15.13%) samples were positive for brucellosis. None of the serologically negative sample showed positivity by PCR; however few positive samples were tested negative by PCR. Sensitivity and specificity of PCR compared with RBPT was 100 and 92.4% while with STAT these were 100 and 95.16%, respectively. Results are promising that whole blood can be used for studying the molecular epidemiology of B. abortus in cattle and particularly detecting the active phase of infection and PCR can be well adopted as a valuable test for mass screening of animals for this purpose. The present study adds to the prevalence data available regarding to B. abortus infection in cattle population and highlights the usefulness and advantages of molecular tool of PCR over serological tests.
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How to cite this article:

K. Karthik, R. Rathore, P. Thomas, A. Elamurugan, T.R. Arun and K. Dhama, 2014. Serological and Molecular Detection of Brucella abortus from Cattle by RBPT, STAT and PCR and Sample Suitability of Whole Blood for PCR. Asian Journal of Animal and Veterinary Advances, 9: 262-269.

DOI: 10.3923/ajava.2014.262.269

URL: https://scialert.net/abstract/?doi=ajava.2014.262.269

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