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Asian Journal of Animal and Veterinary Advances

Year: 2014 | Volume: 9 | Issue: 1 | Page No.: 1-12
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Authors

Shi Shu

Cheng Xia

Hongyou Zhang

Zhaolei Sun

Jiannan Liu

Bo Wang

Keywords

Milk fever is an important metabolic disorder of dairy cows at calving and is characterized by hypocalcemia during the transition period. The aim of this study is to investigate novel changes in the plasma proteomics of cows with milk fever. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was used for many field as a novel proteomics teachnology. So far, the plasma proteomics of milk fever has not been investigated using SELDI-TOF-MS. Plasma samples were obtained from twenty-one Holstein cows with milk fever (T) and fifty-nine Holstein cows without milk fever (C) at a dairy farm in Heilongjiang, China. Twenty-four differential peptide peaks in the plasma of T and C cows were isolated by SELDI-TOF-MS. Ten of these peaks were identified using the Swissport Protein Database. The peptide peaks represented ten unique proteins and showed significant alterations in their peaks as determined by analysis using the Wilcoxon Rank Sum Test. The four up-regulated proteins were identified as complement c3 frag, hepcidin, amyloid bata a4 protein, serum albumin frag and fibrinogen. Complement c3 frag and hepcidin regulate the inflammatory response. Amyloid beta a4 protein is involved in Alzheimer’s disease. Serum albumin frag acts as a transport protein. Fibrinogen beta chain participates in blood coagulation. The two down-regulated proteins were plasma protease c1 inhibitor frag and apolipoprotein a-2 which are associated with, respectively, blood coagulation and cardiovascular disease. The four proteins that were both up-regulated and down-regulated were fibrinogen alpha chain frag, neurosecretory protein vgf frag, serun amyloid a protein and cystatin-c. Based on SELDI-TOF-MS, identify novel plasma proteins that may be closely associates with milk fever in cows. These findings may reveal previously unidentified metabolic changes in cows with milk fever.
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