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Asian Journal of Animal and Veterinary Advances
  Year: 2013 | Volume: 8 | Issue: 6 | Page No.: 833-840
DOI: 10.3923/ajava.2013.833.840
 
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Attempt at Conserving the Genetic Resources of Tan Sheep by Conserving its Fibroblast Line

X.C. Li, P.F. Hu, T.F. Lu, X.H. He, W.J. Guan and Y.H. Ma

Abstract:
Tan sheep is one of the most distinctive local sheep breeds in China, while investigations concerning its cell biology and molecular genetics are still scarce. To preserve the valuable genetic resources of Tan sheep, we established a Tan sheep fibroblast line and identified its biological characteristics, including cell morphology, growth curve, karyotype, isoenzyme polymorphism and expression of exogenous fluorescent genes. The results showed that the viability of the fibroblasts before freezing was 96.75±3.24 and 93.42±2.87% after thawing. The growth curve of Tan sheep ear marginal fibroblasts displayed an obvious “S” shape and there was no microbial contamination and cross-contamination; all somatic chromosomes were acrocentric autosomes and only the two sex chromosomes were submetacentric; the transfection efficiencies of the three fluorescent protein genes were 20.6-36.2%. It could be concluded that the this study has not only provided the biological characteristics of Tan sheep at cellular level but also made a valuable contribution to the preservation of the genetic resources of the Tan sheep.
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How to cite this article:

X.C. Li, P.F. Hu, T.F. Lu, X.H. He, W.J. Guan and Y.H. Ma, 2013. Attempt at Conserving the Genetic Resources of Tan Sheep by Conserving its Fibroblast Line. Asian Journal of Animal and Veterinary Advances, 8: 833-840.

DOI: 10.3923/ajava.2013.833.840

URL: https://scialert.net/abstract/?doi=ajava.2013.833.840

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