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Asian Journal of Animal and Veterinary Advances

Year: 2013 | Volume: 8 | Issue: 6 | Page No.: 827-832
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Authors

Jun He

Xiangyong Qu

Changqing He

Keywords

Research Article

Association Analysis Between Polymorphism of Peroxisome Proliferator-activated Receptor α(PPARα) Gene and Slaughter Performance in Goose

Jun He, Xiangyong Qu and Changqing He
PPAR is one cluster of the most important genes that related with lipid metabolism. And it can used as the index of goose strain selection. In this study, we collected 282 blood samples of 75 days Dongting goose to detect the SNPsof PPARα gene by PCR-SSCP. The results showed that in the gene sequence of 215bp had a mutation of C→T and the gene frequency of A,B were 0.393,0.607, respectively, the genotype frequency of AA, AB, BB were 0.126, 0.533, 0.341, respectively. So we can concluded the polymorphism of goose PPARα gene and the genotype frequency of heterozygote was higher in the test samples. After genotype detection of all samples, 35 AA and 96 BB genotype obtained. The abdominal fat weight of AA and BB genotype were 23.14±5.08 and 43.69±8.04, the abdominal fat ratio and liver weight ratio of AA and BB genotype were 0.80±0.30, 2.74±0.82 and 1.58±0.55, 2.87±0.69, respectively. By statistics of variance, the PPARα genotype was highly significantly associated with abdominal fat weight, abdominal fat ratio and liver weight ratio (p<0.05). In general, the abdominal fat weight, abdominal fat ratio and liver weight ratio showed a tendency of BB>AA, indicating that the common allele was the least favorable for abdominal fat deposition. Thus, there is an enormous opportunity to decrease abdominal fat deposition in case this allele is confirmed in other more studies.
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