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Asian Journal of Animal and Veterinary Advances

Year: 2013 | Volume: 8 | Issue: 5 | Page No.: 723-731
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Authors

Ping Zhao

Ying He

Yuefeng Chu

Baoyu Li

Pengcheng Gao

Xuan Zhang

Haiyan Zhao

Youjun Shang

Zhongxin Lu

Keywords

Mycoplasma capricolum sub sp. capripneumoniae (Mccp) is the causative agent of contagious capripleuropneumonia (CCPP) in goats. To develop more effective vaccines against CCPP, three protein sequences were optimized, in terms of codon usage bias, GC content, restriction enzymes, CIS-acting elements and repeat sequences, which are critical for efficient gene expression. These three proteins, respectively are the pyruvate dehydrogenase E1 component alpha subunit (PDHA), pyruvate dehydrogenase complex E1 component beta subunit (PDHB) and dihydrolipoylysine-residue acetyltransferase components of the pyruvate dehydrogenase complex (PDHC). The three optimized genes were expressed in E. coli and the relevant proteins were analyzed further by in vivo expression detection and antibody detection. The results showed the following: (1) A significantly higher level of serum antibodies against the three proteins were detected with convalescent sera and immunized sera, which demonstrated that the three proteins were expressed in vivo. (2) Goats antisera tests against the three proteins showed that they could all elicit humoral immunity with potential antigenicity and reactogenicity. (3) The results suggested that these three proteins (PDHA, PDHB and PDHC) have strong potential as vaccine candidates.
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How to cite this article

Ping Zhao, Ying He, Yuefeng Chu, Baoyu Li, Pengcheng Gao, Xuan Zhang, Haiyan Zhao, Youjun Shang and Zhongxin Lu, 2013. Optimizing and Expressing of Proteins Including PDHA, PDHB and PDHC of Mycoplasma capricolum sub sp. capripneumoniae. Asian Journal of Animal and Veterinary Advances, 8: 723-731.

DOI: 10.3923/ajava.2013.723.731

URL: https://scialert.net/abstract/?doi=ajava.2013.723.731

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