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Asian Journal of Animal and Veterinary Advances

Year: 2013 | Volume: 8 | Issue: 5 | Page No.: 713-722
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Authors

Meiling Zhang

Tao Gui

Yunsheng Li

Chao Wei

Nian Zhan

Zhen Chen

Yuanliang Zhang

Ya Liu

Qing Jia

Jianping Ding

Xiaorong Zhang

Yunhai Zhang

Keywords

This study was conducted to explore efficient and cost-effective protocols for isolation and culture of mammary epithelial cells from goats during gestation period (non-lactating). Tissues collected from the mammary glands of a Huanghuai white goat, at gestation day 60 were cultured in the presence of fetal bovine serum, insulin-transferrin-selenium and epidermal growth factor. Cell lines were purified using differential enzyme digestion, high density cultivation and continuous passaging. And subsequently characterized by immunofluorescence staining, cell growth curve, oil red O staining and karyotype analysis. In conclusion, a goat mammary epithelial cell line was successfully established and shown to exhibit normal conformation, growth characteristics and lactation function by using improved sample collection and in vitro culture methods. This study provides a new and efficient cell culture protocol, which may facilitate research into the development and function of goat mammary glands and the development of bioreactors.
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How to cite this article

Meiling Zhang, Tao Gui, Yunsheng Li, Chao Wei, Nian Zhan, Zhen Chen, Yuanliang Zhang, Ya Liu, Qing Jia, Jianping Ding, Xiaorong Zhang and Yunhai Zhang, 2013. Establishment and Characterization of a Huanghuai White Goat Mammary Gland Epithelial Cell Line Derived During the Gestational Period. Asian Journal of Animal and Veterinary Advances, 8: 713-722.

DOI: 10.3923/ajava.2013.713.722

URL: https://scialert.net/abstract/?doi=ajava.2013.713.722

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