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Asian Journal of Animal and Veterinary Advances
  Year: 2012 | Volume: 7 | Issue: 10 | Page No.: 940-949
DOI: 10.3923/ajava.2012.940.949
Supplementation Vitrified-thawed Media with Melatonin Do Not Protecting Immature Mouse Testicular Tissue from Vitrified-thawed Induced Injury
Mohammad Reza Gholami, Ghasem Saki, Masoud Hemadi, Ali Khodadadi and Javad Mohammadi-Asl

Abstract:
The purpose of this study was to test of efficacy of melatonin on the Optimizing of cryopreservation media in the testis tissue samples. Testes from neonate BALB/c mice were vitrified and then thawed under standard condition with or without the addition of 100 μM melatonin to both of vitrification and thawing solution. After that, Vitrified-thawed whole testes were digested under standard condition and subsequent viability of the cells in the suspension was analyzed using cytotoxicity kit and Apo-Brdu tunnel assay kit. The mean proportion of apoptotic testicular cells in the treated vitrified-thawed testes in comparison to no-treated ones was noted significantly (5.2±0.47 vs. 1.56±0.62, respectively). Moreover, melatonin cause decreasing the viability of the treated vitrified-thawed testicular cells in compared to no-treated vitrified-thawed testicular cells (4.78±0.46 vs. 8.39±0.76, respectively). In addition, the mean cytotoxicity of melatonin on the vitrified-thawed testicular cells was 9%. The associated reduction in healthy testicular cells in the treated vitrified-thawed testes suggests that melatonin in doses of 100 μM don’t protected testicular tissue from damaged induced in the process vitrification and thawing. However, further well-designed studies such as dosimetry melatonin and applied another cryoprotectants in the matching with melatonin are essential to offer a final conclusion.
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How to cite this article:

Mohammad Reza Gholami, Ghasem Saki, Masoud Hemadi, Ali Khodadadi and Javad Mohammadi-Asl, 2012. Supplementation Vitrified-thawed Media with Melatonin Do Not Protecting Immature Mouse Testicular Tissue from Vitrified-thawed Induced Injury. Asian Journal of Animal and Veterinary Advances, 7: 940-949.

DOI: 10.3923/ajava.2012.940.949

URL: https://scialert.net/abstract/?doi=ajava.2012.940.949

 
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