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Asian Journal of Animal and Veterinary Advances
  Year: 2012 | Volume: 7 | Issue: 3 | Page No.: 273-276
DOI: 10.3923/ajava.2012.273.276
Detection of Coxiella burnetii in Poultry Egg samples in Iran Using Nested PCR Assay
Ebrahim Rahimi and Abbas Doosti

Abstract:
Q-fever is a widespread zoonosis caused by the obligate intracellular microorganism Coxiella burnetii. The epidemiology of Q-fever in Iran is essentially unknown. For this reason, this study for the presence of C. burnetii in hen, duck, goose, quail and ostrich sell egg samples in Isfahan, Gellan and Mazandaran provinces, Iran was conducted. In the present study, 369 hen, duck, goose, quail and ostrich sell egg samples from 15 villages of Isfahan, Gellan and Mazandaran provinces, Iran were tested for C. burnetii using a nested PCR assay. In total, 2 of 130 (1.5%) hen egg samples were positive; the positive samples originated from 1 of 15 (6.6%) villages. Eight of 104 (7.7%) duck egg samples from 2 villages were positive for C. burnetii. As for 34 gooses, 70 quail and 31 ostrich egg samples; they were all tested negative for C. burnetii. To our knowledge, this study is the first report of direct identification of C. burnetii using PCR in hen and duck egg samples in Iran. Further intensive prevalence studies on Coxiella infection and on possible dangers of poultry products will be needed to elucidate the epidemiology of Q-fever in Iran.
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 RELATED ARTICLES:
  •    Q Fever: An Emerging Public Health Concern in Iran
  •    Competitiveness of Polymerase Chain Reaction to Alternate Amplification Methods
  •    Rapid Detection of Bacterial Pathogens in Clinical Body Fluids by Nested PCR
How to cite this article:

Ebrahim Rahimi and Abbas Doosti, 2012. Detection of Coxiella burnetii in Poultry Egg samples in Iran Using Nested PCR Assay. Asian Journal of Animal and Veterinary Advances, 7: 273-276.

DOI: 10.3923/ajava.2012.273.276

URL: https://scialert.net/abstract/?doi=ajava.2012.273.276

 
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