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Asian Journal of Animal and Veterinary Advances
  Year: 2011 | Volume: 6 | Issue: 7 | Page No.: 654-666
DOI: 10.3923/ajava.2011.654.666
 
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Construction of a Recombinant Fowlpox Virus Expressing the E0/E2 Proteins of Classical Swine Fever Virus and its Evaluation in Experimental Animals

Kang-Kang Guo, Wei-Ming Zhang, Yan-Ming Zhang, Peng-Bo Ning, Jing-Yu Wang and Miao-Tao Zhang

Abstract:
Classical Swine Fever (CSF) is a hemorrhagic disease of pigs caused by virulent strains of Classical Swine Fever Virus (CSFV). Vaccination is a useful tool for preventing and controlling CSF. In this study the protective antigens E0 and E2 genes of CSFV were cloned and inserted into a fowlpox virus vector to construct a recombinant plasmid. We transfected this plasmid into chicken embryo cells infected with the fowlpox virus FV282 to package and propagate the recombinant fowlpox virus FV282-E0-E2. Specific anti-CSFV antibodies were detected with enzyme-linked immunosorbent assay in FV282-E0-E2 immunized experimental mice and pigs. Moreover, virus challenge experiments were conducted in experimental pigs immunized with FV282-E0-E2. Immune protection was evaluated in experimental pigs infected with virulent CSFV by observing clinical manifestations and post-mortem examination. The results showed that recombinant fowlpox virus FV282-E0-E2 was obtained after ten cycles of blue plaque selection. The special anti-CSFV antibodies were detected in the experimental mice and pigs. The immunized pigs could resist the attack of the virulent CSFV strain. The recombinant fowlpox virus FV282-E0-E2 represents a potential candidate for the development of a genetic engineered vaccine for the future prevention of CSF.
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How to cite this article:

Kang-Kang Guo, Wei-Ming Zhang, Yan-Ming Zhang, Peng-Bo Ning, Jing-Yu Wang and Miao-Tao Zhang, 2011. Construction of a Recombinant Fowlpox Virus Expressing the E0/E2 Proteins of Classical Swine Fever Virus and its Evaluation in Experimental Animals. Asian Journal of Animal and Veterinary Advances, 6: 654-666.

DOI: 10.3923/ajava.2011.654.666

URL: https://scialert.net/abstract/?doi=ajava.2011.654.666

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