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Asian Journal of Animal and Veterinary Advances
  Year: 2011 | Volume: 6 | Issue: 1 | Page No.: 29-35
DOI: 10.3923/ajava.2011.29.35
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Cryopreservation of Intact and Biopsied Buffalo Blastocysts

Saber Mohamed Abd-Allah

A practical cryopreservation method of embryos is a key factor in commercial embryo transfer and production technology and offers the opportunity of implementing novel animal breeding and production programmes. Initially, work was done with conventional slow cooling technologies, using cryoprotectants, such as glycerol or ethylene glycol and sucrose. More recently, however, greater advances have been made with vitrification, a procedure that bypasses ice crystal formation and places the embryo in a glass-like state. This review will briefly summarize the current understanding of embryo cryopreservation technologies and we strongly believe that we are now eyewitnesses and participants in the creation of a new era of cryopreservation for in vitro produced (IVP) buffalo blastocysts that will be applicable to direct transfer of the post-thaw embryos. Blastocysts were harvested 7 days after IVF and allocated to either an intact or biopsy group. The purpose of the present article is to reflect upon and promote this process.
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How to cite this article:

Saber Mohamed Abd-Allah , 2011. Cryopreservation of Intact and Biopsied Buffalo Blastocysts. Asian Journal of Animal and Veterinary Advances, 6: 29-35.

DOI: 10.3923/ajava.2011.29.35






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