Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Asian Journal of Animal and Veterinary Advances
  Year: 2011 | Volume: 6 | Issue: 3 | Page No.: 242-254
DOI: 10.3923/ajava.2011.242.254
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

A Rapid Way to Map Outheld-Wing Mutation in Drosophila melanogaster

Bo Yu, Huojun Cao, Chunjiang Zhao, Changxin Wu and Xuemei Deng

Abstract:
Map-based positional cloning of Drosophila melanogaster genes is hampered by both the time-consuming, labor-intensive, error-prone nature of traditional methods for genetic mapping-SNP mapping is costly and P element mapping is low-resolution for limit number of P elements. Here we used two strategies to locate a spontaneous mutant screened in our lab. One is P element mapping to map the mutant in a 33 k-interval on the right arm of the second chromosome; another is combination of P element mapping and a Single-Nucleotide Polymorphism (SNP) mapping together to map the mutant in a very small interval, about 5 kb, on the right arm of the second chromosome efficiently. At last, we guessed that the mutant phenotype maybe resulted from the overexpression of twist gene. Comparing the two strategies, the method combining the P insertion mapping and SNP mapping could ward off their drawbacks. Therefore, the strategy of combination of the two methods provides a rapid protocol for mapping mutations.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Differential Sensitivities of Three Strains of Drosophila melanogaster (Diptera: Drosophilidae) to Imipramine as an Antidepressant Drug
How to cite this article:

Bo Yu, Huojun Cao, Chunjiang Zhao, Changxin Wu and Xuemei Deng, 2011. A Rapid Way to Map Outheld-Wing Mutation in Drosophila melanogaster. Asian Journal of Animal and Veterinary Advances, 6: 242-254.

DOI: 10.3923/ajava.2011.242.254

URL: https://scialert.net/abstract/?doi=ajava.2011.242.254

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom