Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Asian Journal of Animal and Veterinary Advances
  Year: 2010 | Volume: 5 | Issue: 8 | Page No.: 557-565
DOI: 10.3923/ajava.2010.557.565
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

The Muscle Transcription Factor MyoD Regulates the Expression of Hepatocyte Growth Factor during the Process of Muscle Development

F. Liu, J. Fei, N. Li and Q. Meng

Abstract:
Muscle regeneration recapitulates myogenesis. HGF (hepatocyte growth factor) plays an important role in muscle development, as the only secreted growth factor, which can activates myoblasts proliferation. The expression units of wild HGF promoter-luciferase vector exceeded the expression units of HGF promoter-luciferase vector with mutated binding sequence of MyoD, also the expression trend of HGF was in accordance with the expression of MyoD. The expression of MyoD directly affects the expression of HGF during muscle development. In C2C12 culture, the expression of HGF is up-regulated in the phase of proliferation process, but down-related in the phase of differentiation. We identified the proliferation and differentiation stage of C2C12 culture by the definition of expression of myogenic regulator family (MyoD, yf5, yogenin, Myf6) and detected the HGF level during this two stages. MyoD bind site was found in the 5’-UTR of HGF promoter. MyoD is b HLH (basic helix-loop-helix) transcriptional activator which has a critical role during myogenesis. We verified that MyoD can bind the active b HLH site, as shown by EMSA. Transfection of wild HGF promoter-luciferase eukaryotic expression vector with MyoD binding site and HGF promoter-luciferase eukaryotic expression vector with mutated MyoD binding site into C2C12 myoblasts was done.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Phylogenetic and Evolutionary Analyses of the Animal Hairy and Enhancer of Split Basic Helix-loop-helix Transcription Factors
How to cite this article:

F. Liu, J. Fei, N. Li and Q. Meng, 2010. The Muscle Transcription Factor MyoD Regulates the Expression of Hepatocyte Growth Factor during the Process of Muscle Development. Asian Journal of Animal and Veterinary Advances, 5: 557-565.

DOI: 10.3923/ajava.2010.557.565

URL: https://scialert.net/abstract/?doi=ajava.2010.557.565

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom