Detection of Avian Influenza Virus Antigen in Chicken Tissues Following Intranasal Inoculation

Research Article

Detection of Avian Influenza Virus Antigen in Chicken Tissues Following Intranasal Inoculation

To understanding the pathogenicity of H9N2 in broiler chickens, the tissue distribution of viral antigen following intranasal (IN) inoculation of this subtype was studied. Eighteen 3-week-old chickens were inoculated with 10⁶EID₅₀ per bird with H9N2 avian influenza virus. Then on days 1, 2, 4, 6, 8 and 11 post-inoculation (PI) samples of the trachea, lung, liver, pancreas, spleen, thymus, duodenum, kidney, brain and bursa of Fabricius were collected for immunofluorescence study. The AIV antigen was detected in the trachea, lung and kidney of inoculated chickens using indirect immunofluorescence technique. The results indicated that the H9N2 avian influenza virus is epitheliotropic in chicken. After IN inoculation it has tissue tropism for trachea, lung (pneumotropic) and kidney (nephrotropic).

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Mohammad Mehdi Hadipour, 2009. Detection of Avian Influenza Virus Antigen in Chicken Tissues Following Intranasal Inoculation. Asian Journal of Animal and Veterinary Advances, 4: 93-98.

DOI: 10.3923/ajava.2009.93.98

URL: https://scialert.net/abstract/?doi=ajava.2009.93.98

Asian Journal of Animal and Veterinary Advances

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Mohammad Mehdi Hadipour

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Research Article

Detection of Avian Influenza Virus Antigen in Chicken Tissues Following Intranasal Inoculation

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