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Transcriptional Profiling of Spleen Lymphocyte in Fowl Typhoid of Broilers |
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H.K. Lim,
K. Choi,
P.K. Mandal,
O. Baatartsogt,
C.H. Lee,
J.H. Lee and H.B. Kim |
Abstract:
This study was carried out to investigate the differentially
expressed genome between S. gallinarum infected and uninfected
control in the spleen lymphocytes of Ross broiler chicks using microarray
analysis. GeneChip Chicken Genome Array containing 32,773 transcripts
corresponding to over 28,000 chicken genes for simultaneous expression
was used. The signal intensity of each gene was normalized and expressed
in fold change. A large numbers of genes were found with differential
expression majority of which are still unknown in chicken genome. Thirty
one known genes were found to have differential expression of which, 25
were up-regulated and 5 were down regulated. Majority of the up-regulated
genes belong to immune response system viz., IL8, IL1B, IL10, IL18,
IL17A, IL15, transferrin, IFNg, TLR2, TNFRSF1b, TNFRSF15 and the down
regulated genes were B-FIV, B-LA, SDF1, B-LBI, belonging to MHC-I and
II and CD1d. To validate the expression of these genes RT-PCR was done
using primers of 12 selected genes′ with total mRNA isolated from
spleen lymphocytes which has confirmed the similar pattern of expression
of all the genes as in microarray. The findings in this study have lead
to the identification of novel genes which may be useful in further studies
to understand the patho-physiology of fowl typhoid towards development
of diagnostics and therapeutics.
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How to cite this article:
H.K. Lim, K. Choi, P.K. Mandal, O. Baatartsogt, C.H. Lee, J.H. Lee and H.B. Kim, 2009. Transcriptional Profiling of Spleen Lymphocyte in Fowl Typhoid of Broilers. Asian Journal of Animal and Veterinary Advances, 4: 66-75.
DOI: 10.3923/ajava.2009.66.75
URL: https://scialert.net/abstract/?doi=ajava.2009.66.75
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