PCR-Based Detection of Yersinia ruckeri Infection in Rainbow Trout Fish

Research Article

**PCR-Based Detection of Yersinia ruckeri Infection in Rainbow Trout Fish**

M.R. Roozbahani, M. Bandehpour, A. Haghighi- Khiabanian-Asl, H. Abdollahi and B. Kazemi

The aim of this study was designing a diagnostic kit for yersiniosis in the trout fish in Iran. Colonies of Yersinia ruckeri were collected from culture medium and a suspension was prepared in a lysing solution. DNA was extracted through a boiling and phenol chloroform method. Two primer sets targeting bacterial 16S rRNA and trout 18S rRNA. Polymerase chain reaction products were separated by gel electrophoresis. Among 20 suspected samples tested two samples were positive for both host and bacterial PCRs indicating the positive Y. ruckeri infection and remaining 18 samples were negative for pathogen. The performance of PCR reactions in negative samples were confirmed from amplification of internal control reactions targeting host. A PCR based diagnostic kit with an internal control was prepared for detection of Yersinia ruckeri in rainbow trout fish.

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How to cite this article

M.R. Roozbahani, M. Bandehpour, A. Haghighi- Khiabanian-Asl, H. Abdollahi and B. Kazemi, 2009. PCR-Based Detection of Yersinia ruckeri Infection in Rainbow Trout Fish. Asian Journal of Animal and Veterinary Advances, 4: 258-262.

DOI: 10.3923/ajava.2009.258.262

URL: https://scialert.net/abstract/?doi=ajava.2009.258.262

Comments

Modares Ravi Roozbahani Reply

17 August, 2009

I would like to thanks becuase of this usefule and practical article . hope to see other article of these scientists. it is exellent

Mohammad Reza Roozbahani

23 December, 2012

That is kind of you friend, Best wishes Roozbahani

Asian Journal of Animal and Veterinary Advances

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Authors

M.R. Roozbahani

M. Bandehpour

A. Haghighi- Khiabanian-Asl

H. Abdollahi

B. Kazemi

Keywords