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Asian Journal of Animal and Veterinary Advances

Year: 2009 | Volume: 4 | Issue: 5 | Page No.: 252-257
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Authors

H. Yardibi

G. Turkay

A. Mengi

F. Kaygysyz

K. Oztabak

Keywords

Research Article

Genetic Polymorphisms of α-lactalbumin and β-lactoglobulin in South Anatolian and East Anatolian Red Cattle

H. Yardibi, G. Turkay, A. Mengi, F. Kaygysyz and K. Oztabak
The objective of the present study was to determine the genotype and allele frequencies for alpha-lactalbumin (α-LA) ve β-lactoglobulin (β-LG) that are claimed to be associated with milk production traits in cattle in South Anatolian Red (SAR) and East Anatolian Red (EAR) cattle. In this study, 40 cattle for each of SAR and EAR were used. Genomic DNA samples were isolated by using standard salt-out method. After Polymerase Chain Reaction (PCR), α-LA and β-LG genes were digested with MspI and RsaI (R5), AvaI (R3), MspI (R1), Sau3A (R2) restriction enzymes, respectively. As a result, SAR and EAR cattle breeds have the lower allel frequencies for α-LA and β-LG gene than high-yielding European dairy cattle breeds. Because of that reason we may claim that applying the selection programs for developing the allels belonging to both genes may contribute to the trialsto improve theproduction parameters in SAR and EAR breed bovines.
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