Submit Manuscript

Asian Journal of Animal and Veterinary Advances

Year: 2008 | Volume: 3 | Issue: 6 | Page No.: 400-408
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 285

Authors

M. Shakeri

H. Roshanfekr

M. Mamoei

Kh. Mirzadeh

Keywords

Research Article

Effect of Epididymis Handling Conditions on the Quality of Ram Spermatozoa Recovered Post-Mortem

M. Shakeri, H. Roshanfekr, M. Mamoei and Kh. Mirzadeh
The present research studied the effect of the interval between animal`s death and sperm recovery (0, 24 or 48h) on the quality and freezability of ram spermatozoa from cauda epididymidis. Storage temperature of epididymes (room temperature or 5°C) was also analyzed. Spermatozoa were diluted with Tes-Tris-Fructose solution supplemented with egg yolk (10%) and glycerol (4%) and frozen using a programmable bio-freezer (-20°C min-1). Pre-freeze and post-thaw sperm samples showed viable spermatozoa up to 48h after the animal`s death, although their quality declined significantly as post-mortem storage time increased. Epididymes sperm stored at 5°C showed better motility and a lower percentage of abnormal forms than epididymes stored at room temperature after 24 and 48h. The fertilizing ability of cauda epididymis ram spermatozoa obtained at 0 and 24h after the animal`s death is similar to that of ejaculated spermatozoa. Therefore, a good protocol for post-mortem semen collection in rams when epididymal spermatozoa cannot be collected immediately is to preserve the epididymes at 5°C and process the samples in the first 24h after the animal`s death.
PDF Fulltext XML References Citation

Leave a Comment

Your email address will not be published. Required fields are marked *