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Asian Journal of Animal and Veterinary Advances
  Year: 2006 | Volume: 1 | Issue: 1 | Page No.: 55-59
DOI: 10.3923/ajava.2006.55.59
 
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Effect of Infectious Bursal Disease Virus on in vitro Propagation of Chicken Embryo Fibroblast Cells

Sarder Nasir Uddin and Sk. A. Hossain

Abstract:
The present research was undertaken to study the propagation and observation of cytopathic effect of IBDV in chicken embryo fibroblast cells. Chicken Embryo Fibroblast (CEF) is chicken embryo derived primary cell culture. Infectious bursal disease is an acute, highly contagious viral disease of young chickens caused by a double stranded RNA virus named Infectious Bursal Disease Virus (IBDV). For this purpose, suspected IBDV isolates were collected from the bursas of dead chicken of a particular flock. Local field IBDV isolates was then inoculated in chicken embryo for slight adaptation by several passages. CEF cells was prepared from 9-10 days chicken embryo and transferred in monolayer culture flasks containing maintenance media with 1-2% heat-inactivated fetal calf serum. Adapted IBDV was then inoculated in CEF cell culture for the purpose of propagation. After inoculation characteristics clear and consistent Cytopathic Effects (CPEs) were observed on CEF cell monolayer and after 72 h of infection, the cells were started to change its shape.
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How to cite this article:

Sarder Nasir Uddin and Sk. A. Hossain , 2006. Effect of Infectious Bursal Disease Virus on in vitro Propagation of Chicken Embryo Fibroblast Cells. Asian Journal of Animal and Veterinary Advances, 1: 55-59.

DOI: 10.3923/ajava.2006.55.59

URL: https://scialert.net/abstract/?doi=ajava.2006.55.59

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