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Asian Journal of Animal Sciences
  Year: 2021 | Volume: 15 | Issue: 1 | Page No.: 35-42
DOI: 10.3923/ajas.2021.35.42
 
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Genetic Relationship among Three Nigerian Chicken (Gallus gallus) Genotypes Based on Cytochrome b of Mitochondrial DNA

E.V. Ikpeme, E.E. Ekerette , I.E. Job, A.J. Umoyen, P.B. Osim and M.O. Ozoje

Abstract:
Background and Objective: Demand for poultry and livestock products in general has increased significantly because of the dietary importance of meat and egg that they produced. In this study, the genetic relationship of three local chicken breeds in South-South Nigeria (frizzle feather, normal feather and naked neck) was evaluated. Materials and Methods: Two milliliter of blood was collected from their wing vein using sterile needle and syringe and stored in FTA cards for DNA extraction, PCR amplification and sequencing. Results: The result obtained revealed a total of 9 haplotypes. Frizzle feather had the highest haplotype and nucleotide diversity while the lowest haplotype and nucleotide diversity was from naked neck. High sequence conservation was observed across the three genotypes. Genetic distance estimates revealed that naked neck and frizzle feather were more closely related while the furthest genetic distance was between normal feather and frizzle feather. The phylogenetic tree revealed two clusters. Cluster one had three individuals from the same population (normal feather). All other samples were grouped under cluster 2. Conclusion: The study revealed the existence of low genetic diversity among the three populations of chicken studied and also showed that the Cytochrome b gene is highly conserved.
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How to cite this article:

E.V. Ikpeme, E.E. Ekerette, I.E. Job, A.J. Umoyen, P.B. Osim and M.O. Ozoje, 2021. Genetic Relationship among Three Nigerian Chicken (Gallus gallus) Genotypes Based on Cytochrome b of Mitochondrial DNA. Asian Journal of Animal Sciences, 15: 35-42.

DOI: 10.3923/ajas.2021.35.42

URL: https://scialert.net/abstract/?doi=ajas.2021.35.42

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